Muc10 (BC021401) Mouse Tagged ORF Clone

CAT#: MR201463

Muc10 (Myc-DDK-tagged) - Mouse mucin 10, submandibular gland salivary mucin (cDNA clone MGC:29112 IMAGE:4166946)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Muc10
• Synonyms: Muc10
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR201463 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAAATGAAATGTTCATCTTGGGCCTGTTGGCTCTTACTTCATATTTCATGCCTGGTGAAAGTCATC
  ACAAGTCAAGAAGTTCACAATTCCCTGTCAGAAAATATCTAGAAGACCCAAGATATCCAAGATATCCAAG
  ACCACATTATTCCTATGGTTTCATTCCAAGCTCTCCAAAGTTTCCAAAAGACAATCAATGGTATAAGATG
  TGCCCTCCAGGAACTACCTTAATGCTGATCAGTCGCCGTTCTCCTAAATTTCTATGCATTCCTAAAAGAC
  AGATTATCTCTGATAAAACGAAACCAAATGCAACCACACCAGCAACAACCACCAAATCTACAACAAAAGA
  ACCTACTACTTCACCTAAGCCTAGCACCTCTACAGCCATACCTACAACAACTAAGTCTGCAAATAGTACT
  TCCTCTACTACAACTAGTACTACCATCCAAACTACAGCTCCAACATTTGCGGAAGTGTTTTGGAAGTTCT
  TTCAGCAGATCTTTAGGTTAAAGAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR201463 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKNEMFILGLLALTSYFMPGESHHKSRSSQFPVRKYLEDPRYPRYPRPHYSYGFIPSSPKFPKDNQWYKM
  CPPGTTLMLISRRSPKFLCIPKRQIISDKTKPNATTPATTTKSTTKEPTTSPKPSTSTAIPTTTKSANST
  SSTTTSTTIQTTAPTFAEVFWKFFQQIFRLKK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC021401
• ORF Size: 516 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC021401, AAH21401
• RefSeq Size: 757 bp
• RefSeq ORF: 518 bp
• Locus ID: 17830
• MW: 19.6 kDa