Sip1 (BC053424) Mouse Tagged ORF Clone

CAT#: MR201583

Sip1 (Myc-DDK-tagged) - Mouse survivor of motor neuron protein interacting protein 1 (cDNA clone MGC:59266 IMAGE:6336522)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Sip1
• Synonyms: Gemin2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR201583 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTATGTATTACTTAATTGTAGGATCGAAGCAGCCCAATGTCCAGATGTTGTGGTAGCCCAGATTGACC
  CAAAGAAGTTGAAAAGGAAGCAAAGTGTGAACATTTCTGTTCAGAGATTCAGTCCATTATCATCAAGGTG
  GGAGCATGGCAGTATCCAGGCAGGCTTGGCACAGGAGGAACTGAGAGTTCTACATCTTCATCCAAAGGCT
  GCTAGTGGAAGACTGACTCCCAGGCAACTAGGCTTTCCGGATGCCAGCCTGCGCCTGAAGGTTACTCTCC
  AACACTTCAGTGGCAACAACAACAAGTGGCACATTTTTCAACTGTTCGACAGAGTGTACACAAGCATAGA
  AATCACTGGAAATCACAACAGTTGGACAGTAATGTGGCAATGCCAAAATCTGAAGATGAAGAAGGCTGGA
  AAAAATTTTGTCTGGGTGAAAGGTTATGTGCTGAAGGGGCCACTGGACCGTCTACAGAGGAAAGCCCTGG
  GATCGATTATGTACAAGTTGGTTTTCCTCCTTTGCTTAGTATTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR201583 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MYVLLNCRIEAAQCPDVVVAQIDPKKLKRKQSVNISVQRFSPLSSRWEHGSIQAGLAQEELRVLHLHPKA
  ASGRLTPRQLGFPDASLRLKVTLQHFSGNNNKWHIFQLFDRVYTSIEITGNHNSWTVMWQCQNLKMKKAG
  KNFVWVKGYVLKGPLDRLQRKALGSIMYKLVFLLCLVL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC053424
• ORF Size: 534 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC053424, AAH53424
• RefSeq Size: 2064
• RefSeq ORF: 536
• Locus ID: 66603
• MW: 20.4 kDa
• Gene Summary: This gene encodes one of the proteins found in the survival of motor neuron (SMN) complex, which consists of the SMN protein and several gemin proteins. The SMN complex is localized to a subnuclear compartment called gems (gemini of coiled bodies) and is required for assembly of spliceosomal small nuclear ribonucleoproteins (snRNP) and for pre-mRNA splicing. This protein interacts directly with the SMN protein and it is required for formation of the SMN complex. Disruption of this gene in mouse resulted in impaired snRNP assembly, and motor neuron degeneration. [provided by RefSeq, Sep 2015]