Pla2g12a (BC026812) Mouse Tagged ORF Clone

CAT#: MR201855

Pla2g12a (Myc-DDK-tagged) - Mouse phospholipase A2, group XIIA (cDNA clone MGC:25458 IMAGE:4456431)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pla2g12a
• Synonyms: mGXII-1-PLA2, MGC58884, GXII, mGXII-1, Rossy
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR201855 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGACTCCGCGGCCCGCGCCCGCCCGGGGCCCCGCGCTCCTCCTCCTCCTGCTGCTGGCCACTGCGC
  GCGGGCAGGAACAGGACCAGACCACCGACTGGAGGGCCACCCTCAAGACCATCCGCAACGGCATCCACAA
  GATAGACACGTACCTCAACGCCGCGCTGGACCTGCTGGGCGGGGAGGACGGGCTCTGCCAGTACAAGTGC
  AGCGACGGATCGAAGCCTGTTCCACGCTATGGATATAAACCATCTCCACCAAATGGCTGTGGCTCTCCAC
  TGTTTGGCGTTCATCTGAACATAGGTATCCCTTCCCTGACCAAGTGCTGCAACCAGCACGACAGATGCTA
  TGAGACCTGCGGGAAAAGCAAGAACGACTGTGACGAGGAGTTCCAGTACTGCCTCTCCAAGATCTGCAGA
  GACGTGCAGAAGACGCTCGGACTATCTCAGAACGTCCAGGCATGTGAGACAACGGTGGAGCTCCTCTTTG
  ACAGCGTCATCCATTTAGGCTGCAAGCCATACCTGGACAGCCAGCGGGCTGCATGCTGGTGTCGTTATGA
  AGAAAAAACAGATCTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR201855 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVTPRPAPARGPALLLLLLLATARGQEQDQTTDWRATLKTIRNGIHKIDTYLNAALDLLGGEDGLCQYKC
  SDGSKPVPRYGYKPSPPNGCGSPLFGVHLNIGIPSLTKCCNQHDRCYETCGKSKNDCDEEFQYCLSKICR
  DVQKTLGLSQNVQACETTVELLFDSVIHLGCKPYLDSQRAACWCRYEEKTDL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC026812
• ORF Size: 576 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC026812, AAH26812
• RefSeq Size: 1022 bp
• RefSeq ORF: 578 bp
• Locus ID: 66350
• MW: 21.3 kDa