Ak1 (BC014802) Mouse Tagged ORF Clone

CAT#: MR201878

Ak1 (Myc-DDK-tagged) - Mouse adenylate kinase 1 (cDNA clone MGC:25242 IMAGE:4505142)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ak1
• Synonyms: Ak-1|B430205N08Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR201878 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGAGAAGCTGAAGAAGGCCAAGATCATCTTTGTGGTGGGCGGACCTGGCTCAGGAAAGGGCACCC
  AGTGCGAGAAGATTGTACAGAAATATGGCTACACCCACCTGTCTACTGGGGACCTGCTTCGGGCAGAAGT
  CAGCTCTGGATCGGAGAGGGGCAAGAAGCTGTCGGCTATCATGGAGAAGGGAGAGCTAGTGCCACTGGAC
  ACGGTGCTGGACATGCTCCGAGATGCTATGTTAGCCAAAGTGGATTCTTCCAATGGCTTCCTGATCGACG
  GCTACCCGAGGGAGGTGAAACAGGGAGAAGAATTTGAACAGAAGATTGGACAGCCCACACTGCTGCTGTA
  TGTGGACGCAGGCGCCGAGACCATGACCCAACGACTCCTGAAGCGAGGGGAGACCAGTGGCCGCGTGGAT
  GACAACGAGGAGACCATCAAGAAGCGGCTGGAGACTTATTACAATGCCACAGAACCTGTCATCTCCTTCT
  ATGACAAGCGTGGCATTGTGCGCAAGGTCAATGCCGAAGGCACGGTGGACACTGTCTTCTCTGAGGTCTG
  CACCTATCTTGACTCCCTGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR201878 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEEKLKKAKIIFVVGGPGSGKGTQCEKIVQKYGYTHLSTGDLLRAEVSSGSERGKKLSAIMEKGELVPLD
  TVLDMLRDAMLAKVDSSNGFLIDGYPREVKQGEEFEQKIGQPTLLLYVDAGAETMTQRLLKRGETSGRVD
  DNEETIKKRLETYYNATEPVISFYDKRGIVRKVNAEGTVDTVFSEVCTYLDSLK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC014802
• ORF Size: 582 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC014802, AAH14802
• RefSeq Size: 2059 bp
• RefSeq ORF: 584 bp
• Locus ID: 11636
• MW: 21.5 kDa