Gfer (NM_023040) Mouse Tagged ORF Clone

CAT#: MR201982

Gfer (Myc-DDK-tagged) - Mouse growth factor, erv1 (S. cerevisiae)-like (augmenter of liver regeneration) (Gfer)

Product Images

Coomassie blue staining of purified Gfer protein (Cat# TP501982). The protein was produced from HEK293T cells transfected with Gfer cDNA clone (Cat# MR201982) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gfer
• Synonyms: Alr; ERV1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR201982 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGCCCAGCGAGCCGGCGGGCTTCCCTCGCGGCAGTCGCTTCTCCTTCCTGCCGGGCGGCGCGC
  GCTCCGAGATGACCGACGACCTGGTGACTGACGCGCGGGGCCGCGGCGCAAGGCATAGAGACGACACCAC
  CCCTTCCGCGGCCCCGGCGCCGCAAGGTTTGGAGCACGGGAAGCGACCGTGCCGGGCCTGCGTGGACTTC
  AAGTCGTGGATGCGGACCCAGCAGAAGCGGGACATCAAGTTTAGGGAGGACTGTCCGCAGGATCGGGAAG
  AATTGGGTCGCCACACCTGGGCTTTCCTCCATACGCTGGCCGCCTATTACCCGGACAGGCCCACGCCAGA
  ACAACAACAGGATATGGCCCAGTTCATACATATATTTTCCAAGTTTTACCCCTGCGAGGAATGTGCGGAA
  GACATAAGGAAGAGGATAGGCAGGAACCAGCCAGACACAAGCACTCGAGTATCCTTCAGCCAGTGGCTGT
  GCCGCCTGCACAATGAGGTGAATCGGAAGCTGGGCAAGCCTGATTTTGACTGCTCGAGAGTAGATGAGCG
  TTGGCGTGACGGCTGGAAGGACGGCTCCTGTGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR201982 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAPSEPAGFPRGSRFSFLPGGARSEMTDDLVTDARGRGARHRDDTTPSAAPAPQGLEHGKRPCRACVDF
  KSWMRTQQKRDIKFREDCPQDREELGRHTWAFLHTLAAYYPDRPTPEQQQDMAQFIHIFSKFYPCEECAE
  DIRKRIGRNQPDTSTRVSFSQWLCRLHNEVNRKLGKPDFDCSRVDERWRDGWKDGSCD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_023040
• ORF Size: 597 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_023040.2, NM_023040.3, NP_075527.2
• RefSeq Size: 1775 bp
• RefSeq ORF: 597 bp
• Locus ID: 11692
• MW: 22.9 kDa