Rab5a (BC004842) Mouse Tagged ORF Clone

CAT#: MR202370

Rab5a (Myc-DDK-tagged) - Mouse RAB5A, member RAS oncogene family (cDNA clone MGC:6509 IMAGE:2649317)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rab5a
• Synonyms: 2410015H04Rik|AI663973|AU021172|nnyRab5a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR202370 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTAATCGAGGAGCAACAAGACCCAACGGGCCAAATACTGGAAATAAAATATGCCAGTTCAAACTGG
  TCCTTCTAGGAGAGTCTGCTGTTGGCAAATCAAGCCTGGTTCTTCGCTTTGTGAAAGGCCAATTTCATGA
  ATTTCAAGAGAGTACCATTGGGGCTGCCTTTCTAACCCAAACTGTGTGTCTTGATGACACAACAGTAAAA
  TTTGAAATATGGGATACAGCTGGTCAAGAACGGTATCATAGCTTAGCACCAATGTACTACCGAGGAGCAC
  AAGCAGCCATAGTTGTGTATGATATCACAAATGAGGAATCCTTTGCGAGAGCAAAAAACTGGGTTAAAGA
  ACTTCAAAGGCAAGCAAGTCCTAATATTGTGATAGCTTTGTCAGGAAACAAAGCTGACTTAGCAAATAAA
  AGAGCTGTTGACTTCCAGGAAGCACAGTCCTATGCAGATGACAACAGCTTATTATTTATGGAGACATCAG
  CTAAGACATCAATGAATGTAAATGAAATATTTATGGCAATAGCTAAAAAGCTGCCAAAGAATGAACCACA
  GAATCCTGGTGCAAACTCAGCCAGAGGACGAGGAGTAGACCTTACTGAGCCTGCACAGCCAGCCAGAAGC
  CAGTGTTGTAGTAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR202370 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MANRGATRPNGPNTGNKICQFKLVLLGESAVGKSSLVLRFVKGQFHEFQESTIGAAFLTQTVCLDDTTVK
  FEIWDTAGQERYHSLAPMYYRGAQAAIVVYDITNEESFARAKNWVKELQRQASPNIVIALSGNKADLANK
  RAVDFQEAQSYADDNSLLFMETSAKTSMNVNEIFMAIAKKLPKNEPQNPGANSARGRGVDLTEPAQPARS
  QCCSN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC004842
• ORF Size: 645 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC004842.1
• RefSeq Size: 2246 bp
• RefSeq ORF: 647 bp
• Locus ID: 271457
• MW: 23.6 kDa