Wif1 (BC004048) Mouse Tagged ORF Clone

CAT#: MR202510

Wif1 (Myc-DDK-tagged) - Mouse Wnt inhibitory factor 1 (cDNA clone MGC:7686 IMAGE:3497155)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Wif1
• Synonyms: AW107799|WIF-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR202510 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCAGCCCAGCCTGACCTCAAGGGCACGTCTTTACGCCTTGTTTCTCTCTGGTGCAGCTGAGTGTC
  CCGGAGGGTGTCGAAATGGAGGCTTTTGTAACGAAAGGCGGGTCTGCGAGTGTCCGGATGGGTTCTACGG
  GCCTCACTGTGAGAAAGCCCTGTGCATACCCCGATGTATGAACGGTGGTCTGTGTGTCACTCCTGGCTTC
  TGCATCTGCCCCCCTGGATTCTACGGTGTCAACTGTGACAAAGCAAACTGCTCAACCACCTGCTTTAATG
  GAGGGACCTGCTTTTACCCGGGAAAATGTATTTGCCCTCCTGGACTCGAGGGAGAGCAGTGTGAACTCAG
  CAAATGCCCCCAACCCTGCCGAAATGGAGGTAAATGCATTGGTAAAAGCAAGTGTAAGTGCCCGAAAGGT
  TACCAAGGAGACCTGTGCTCTAAGCCCGTCTGCGAGCCTGGCTGTGGTGCCCACGGAACCTGCCACGAAC
  CCAACAAGTGCCAGTGTCGAGAGGGCTGGCACGGCAGACACTGCAATAAGAGGTATGGAGCCAGCCTCAT
  GCATGCCCCGAGGCCAGCAGGCGCCGGGCTGGAGCGACACACGCCTTCACTTAAAAAGGCTGAGGATAGA
  AGGGATCCACCTGAATCCAATTACATCTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR202510 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAQPSLTSRARLYALFLSGAAECPGGCRNGGFCNERRVCECPDGFYGPHCEKALCIPRCMNGGLCVTPGF
  CICPPGFYGVNCDKANCSTTCFNGGTCFYPGKCICPPGLEGEQCELSKCPQPCRNGGKCIGKSKCKCPKG
  YQGDLCSKPVCEPGCGAHGTCHEPNKCQCREGWHGRHCNKRYGASLMHAPRPAGAGLERHTPSLKKAEDR
  RDPPESNYIW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC004048
• ORF Size: 660 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC004048, AAH04048
• RefSeq Size: 1526 bp
• RefSeq ORF: 662 bp
• Locus ID: 24117
• MW: 23.7 kDa
• Gene Summary: Binds to WNT proteins and inhibits their activities. May be involved in mesoderm segmentation. [UniProtKB/Swiss-Prot Function]