Wif1 (BC004048) Mouse Tagged ORF Clone

CAT#: MR202510

  • TrueORF®

Wif1 (Myc-DDK-tagged) - Mouse Wnt inhibitory factor 1 (cDNA clone MGC:7686 IMAGE:3497155)


  "BC004048" in other vectors (3)


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Reconstitution Protocol

USD 68.00

USD 310.00

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Size
    • 10 ug

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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Wif1
Synonyms AW107799|WIF-1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR202510 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTCAGCCCAGCCTGACCTCAAGGGCACGTCTTTACGCCTTGTTTCTCTCTGGTGCAGCTGAGTGTC
CCGGAGGGTGTCGAAATGGAGGCTTTTGTAACGAAAGGCGGGTCTGCGAGTGTCCGGATGGGTTCTACGG
GCCTCACTGTGAGAAAGCCCTGTGCATACCCCGATGTATGAACGGTGGTCTGTGTGTCACTCCTGGCTTC
TGCATCTGCCCCCCTGGATTCTACGGTGTCAACTGTGACAAAGCAAACTGCTCAACCACCTGCTTTAATG
GAGGGACCTGCTTTTACCCGGGAAAATGTATTTGCCCTCCTGGACTCGAGGGAGAGCAGTGTGAACTCAG
CAAATGCCCCCAACCCTGCCGAAATGGAGGTAAATGCATTGGTAAAAGCAAGTGTAAGTGCCCGAAAGGT
TACCAAGGAGACCTGTGCTCTAAGCCCGTCTGCGAGCCTGGCTGTGGTGCCCACGGAACCTGCCACGAAC
CCAACAAGTGCCAGTGTCGAGAGGGCTGGCACGGCAGACACTGCAATAAGAGGTATGGAGCCAGCCTCAT
GCATGCCCCGAGGCCAGCAGGCGCCGGGCTGGAGCGACACACGCCTTCACTTAAAAAGGCTGAGGATAGA
AGGGATCCACCTGAATCCAATTACATCTGG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR202510 protein sequence
Red=Cloning site Green=Tags(s)

MAQPSLTSRARLYALFLSGAAECPGGCRNGGFCNERRVCECPDGFYGPHCEKALCIPRCMNGGLCVTPGF
CICPPGFYGVNCDKANCSTTCFNGGTCFYPGKCICPPGLEGEQCELSKCPQPCRNGGKCIGKSKCKCPKG
YQGDLCSKPVCEPGCGAHGTCHEPNKCQCREGWHGRHCNKRYGASLMHAPRPAGAGLERHTPSLKKAEDR
RDPPESNYIW

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC004048
ORF Size 660 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq BC004048, AAH04048
RefSeq Size 1526 bp
RefSeq ORF 662 bp
Locus ID 24117
MW 23.7 kDa
Gene Summary Binds to WNT proteins and inhibits their activities. May be involved in mesoderm segmentation. [UniProtKB/Swiss-Prot Function]

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