Crp (NM_007768) Mouse Tagged ORF Clone

CAT#: MR202645

Crp (Myc-DDK-tagged) - Mouse C-reactive protein, pentraxin-related (Crp)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Crp
• Synonyms: AI255847
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR202645 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAAGCTACTCTGGTGCCTTCTGATCATGATCAGCTTCTCTCGGACTTTTGGTCATGAAGACATGT
  TTAAAAAGGCCTTTGTATTTCCCAAGGAGTCAGATACTTCCTATGTGTCTCTGGAAGCAGAGTCAAAGAA
  GCCACTGAACACCTTTACTGTGTGTCTCCATTTCTACACTGCTCTGAGCACAGTGCGCAGCTTCAGTGTC
  TTCTCTTATGCTACCAAGAAGAACTCTAACGACATTCTCATATTTTGGAATAAGGATAAACAGTATACTT
  TTGGAGTGGGTGGTGCTGAAGTACGATTCATGGTTTCAGAGATTCCTGAGGCTCCAACACACATCTGTGC
  CAGCTGGGAGTCTGCTACGGGGATTGTAGAGTTCTGGATTGATGGGAAACCCAAGGTGCGGAAAAGTCTG
  CACAAGGGCTACACTGTGGGGCCAGATGCAAGCATCATCTTGGGGCAGGAGCAGGACTCGTATGGCGGTG
  ACTTTGATGCAAAGCAGTCTTTGGTGGGAGACATCGGAGATGTGAACATGTGGGATTTTGTGCTATCTCC
  AGAACAGATCAACACAGTCTATGTTGGTGGGACACTCAGCCCCAATGTTTTGAACTGGCGGGCACTGAAC
  TATAAAGCACAGGGTGATGTGTTTATTAAGCCGCAGCTGTGGTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR202645 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEKLLWCLLIMISFSRTFGHEDMFKKAFVFPKESDTSYVSLEAESKKPLNTFTVCLHFYTALSTVRSFSV
  FSYATKKNSNDILIFWNKDKQYTFGVGGAEVRFMVSEIPEAPTHICASWESATGIVEFWIDGKPKVRKSL
  HKGYTVGPDASIILGQEQDSYGGDFDAKQSLVGDIGDVNMWDFVLSPEQINTVYVGGTLSPNVLNWRALN
  YKAQGDVFIKPQLWS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007768
• ORF Size: 678 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007768.1, NM_007768.2, NM_007768.3, NM_007768.4, NP_031794.2
• RefSeq Size: 1698 bp
• RefSeq ORF: 678 bp
• Locus ID: 12944
• MW: 25.4 kDa
• Gene Summary: Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. [UniProtKB/Swiss-Prot Function]