Nrl (NM_008736) Mouse Tagged ORF Clone

CAT#: MR202892

Nrl (Myc-DDK-tagged) - Mouse neural retina leucine zipper gene (Nrl), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nrl
• Synonyms: D14H14S46E
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR202892 representing NM_008736
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTTCCCTCCCAGTCCCTTGGCTATGGAATATGTTAATGACTTTGATTTGATGAAGTTCGAAATAA
  AGCGGGAGCCTTCTGAGGGCCGATCTGGAGTCCCCACAGCCTCGTTGGGCTCCACACCATACAGCTCGGT
  GCCTCCTTCACCCACCTTCAGTGAGCCAGGCATGGTGGGTGGTGGCGAGGCCCCTAGGCCAGGCCTGGAG
  GAGCTATATTGGCTGGCCACCCTGCAGCAGCAGCTGGGGTCGGATGAGGTTCTCGGGCTGAGTCCCGACG
  AAGCTGTGGAGCTGCTGCAGAACCAGGGTCCTGTCTCTATGGAAGGGCCTCTTGGCTACTATTCAGGGAG
  CCCGGGAGAGACAGGAGCCCAGCATGTCCAGCTGCCCGAGAGATTTTCGGACGCCGCGCTGGTCTCGATG
  TCTGTGCGCGAGTTGAACCGGCAGCTGCGGGGCTGCGGGCGCGACGAGGCTCTGCGGCTGAAGCAGAGGC
  GCCGCACGCTCAAGAACCGCGGCTACGCTCAGGCTTGTCGCTCCAAGCGGCTGCAACAGCGGCGCGGGCT
  GGAGGCAGAGCGCGCTCGCCTGGCCGCCCAGCTGGATGCCCTGCGGGCCGAGGTGGCACGCCTGGCTCGC
  GAGCGCGACCTCTATAAGGCCCGCTGTGACCGGCTGACCTCCGGCGGCCCTGGGTCCGACGACCACACAC
  ACCTCTTCCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR202892 representing NM_008736
  Red=Cloning site Green=Tags(s)
  
  MAFPPSPLAMEYVNDFDLMKFEIKREPSEGRSGVPTASLGSTPYSSVPPSPTFSEPGMVGGGEAPRPGLE
  ELYWLATLQQQLGSDEVLGLSPDEAVELLQNQGPVSMEGPLGYYSGSPGETGAQHVQLPERFSDAALVSM
  SVRELNRQLRGCGRDEALRLKQRRRTLKNRGYAQACRSKRLQQRRGLEAERARLAAQLDALRAEVARLAR
  ERDLYKARCDRLTSGGPGSDDHTHLFL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008736
• ORF Size: 711 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008736.1, NM_008736.2, NM_008736.3, NP_032762.1
• RefSeq Size: 2422 bp
• RefSeq ORF: 714 bp
• Locus ID: 18185
• MW: 26.5 kDa
• Gene Summary: This gene encodes a member of the basic leucine zipper domain family of transcription factors. The encoded protein is preferentially expressed in the retina and is necessary for rod photoreceptor development. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2012]