Dcun1d5 (NM_029775) Mouse Tagged ORF Clone

CAT#: MR202903

Dcun1d5 (Myc-DDK-tagged) - Mouse DCN1, defective in cullin neddylation 1, domain containing 5 (S. cerevisiae) (Dcun1d5)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dcun1d5
• Synonyms: 3110001A18Rik; 4833420K19Rik; AW060460; D430047L21Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR202903 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAGTGAAGAAGAAGAGAAAAGCTCCCGGAGTGGCAGCGGCGGTCGCGGAAGACGCGGGCCTCAAAA
  AGTGTAAAATCCCCAGCTATTGCAGATCCCAGCCTCCTGCTAGACTCATCAGTGGAGAGGAAGATTTTTC
  AAGAAAGAAGTGCCTGGCTTGGTTTTATGAGTATGCAGGTCCTGATGAAGTTGTAGGGCCAGAAGGAATG
  GAAAAATTTTGTGAAGACATTGGTGTTGAACCTGAAAACATTATTATGTTAGTTTTAGCATGGAAATTGG
  AGGCTGAAAGCATGGGATTTTTTACCAAGGAAGAATGGTTAAAGGGAATGACCTCATTACAGTGTGACTG
  CACAGAAAAGTTACAGAGCAGGTTTGATTTTCTGCGCTCTCAGCTGAATGACATCTCATCTTTTAAGAAT
  ATCTACAGATATGCCTTTGATTTTGCAAGGGATAAAGATCAGAGAAGCCTTGATATTGATACTGCGAAGT
  CTATGTTAGCTCTGCTGCTTGGGAGAACATGGCCACTCTTTTCAGTATTTTACCAGTACCTGGAGCAATC
  AAAGTATCGTGTTATGAACAAAGATCAGTGGTACAATGTATTAGAATTCAGCAGAACAGTTCATGCCGAT
  CTTAGTAACTACGATGAAGACGGTGCTTGGCCTGTTCTTCTTGATGAATTTGTTGAGTGGCAAAAAATCC
  GTCAAACATCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR202903 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPVKKKRKAPGVAAAVAEDAGLKKCKIPSYCRSQPPARLISGEEDFSRKKCLAWFYEYAGPDEVVGPEGM
  EKFCEDIGVEPENIIMLVLAWKLEAESMGFFTKEEWLKGMTSLQCDCTEKLQSRFDFLRSQLNDISSFKN
  IYRYAFDFARDKDQRSLDIDTAKSMLALLLGRTWPLFSVFYQYLEQSKYRVMNKDQWYNVLEFSRTVHAD
  LSNYDEDGAWPVLLDEFVEWQKIRQTS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_029775
• ORF Size: 714 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029775.1, NM_029775.2, NP_084051.1
• RefSeq Size: 1201 bp
• RefSeq ORF: 714 bp
• Locus ID: 76863
• MW: 27.6 kDa