C1qc (NM_007574) Mouse Tagged ORF Clone

CAT#: MR203092

  • TrueORF®

C1qc (Myc-DDK-tagged) - Mouse complement component 1, q subcomponent, C chain (C1qc)


  "NM_007574" in other vectors (4)


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Reconstitution Protocol

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USD 149.00

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Size
    • 10 ug

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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol C1qc
Synonyms Adib; AI385742; C1qg; Ciqc
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR203092 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTCGTTGGACCCAGTTGCCAGCCTCCATGTGGACTTTGCCTGCTGCTGCTGTTTCTTCTGGCCCTAC
CACTCAGGAGCCAGGCCAGCGCTGGCTGCTATGGGATCCCAGGGATGCCAGGCATGCCGGGGGCCCCTGG
GAAGGACGGGCATGATGGACTCCAGGGGCCCAAGGGAGAGCCAGGAATCCCAGCCGTCCCTGGGACCCGA
GGACCCAAGGGTCAGAAGGGCGAGCCTGGCATGCCTGGCCACCGTGGGAAAAATGGCCCTAGGGGGACCT
CAGGGTTGCCAGGGGACCCAGGCCCCAGGGGGCCTCCGGGGGAGCCAGGTGTGGAGGGCCGATACAAACA
GAAGCACCAGTCGGTATTCACAGTCACCCGGCAGACCACCCAGTACCCAGAGGCCAACGCCCTCGTCAGG
TTCAACTCTGTGGTCACCAACCCTCAGGGGCATTACAACCCAAGCACAGGGAAGTTCACCTGTGAAGTGC
CGGGCCTCTACTACTTCGTCTACTACACATCGCATACGGCCAACCTGTGCGTGCACCTGAACCTCAACCT
TGCCAGGGTGGCCAGCTTCTGCGACCACATGTTCAACAGCAAGCAGGTCAGCTCCGGAGGAGTCCTCCTG
CGGCTCCAGAGGGGCGACGAGGTGTGGCTATCAGTCAATGACTACAATGGCATGGTGGGCATAGAGGGCT
CCAACAGCGTCTTCTCTGGTTTCCTACTGTTTCCCGAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR203092 protein sequence
Red=Cloning site Green=Tags(s)

MVVGPSCQPPCGLCLLLLFLLALPLRSQASAGCYGIPGMPGMPGAPGKDGHDGLQGPKGEPGIPAVPGTR
GPKGQKGEPGMPGHRGKNGPRGTSGLPGDPGPRGPPGEPGVEGRYKQKHQSVFTVTRQTTQYPEANALVR
FNSVVTNPQGHYNPSTGKFTCEVPGLYYFVYYTSHTANLCVHLNLNLARVASFCDHMFNSKQVSSGGVLL
RLQRGDEVWLSVNDYNGMVGIEGSNSVFSGFLLFPD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_007574
ORF Size 741 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_007574.1, NM_007574.2, NP_031600.2
RefSeq Size 1063 bp
RefSeq ORF 741 bp
Locus ID 12262
MW 26 kDa
Gene Summary C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes. [UniProtKB/Swiss-Prot Function]

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