Gzmb (NM_013542) Mouse Tagged ORF Clone

CAT#: MR203106

Gzmb (Myc-DDK-tagged) - Mouse granzyme B (Gzmb)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gzmb
• Synonyms: AI553453; CCP-1/C11; CCP1; Ctla-1; Ctla1; GZB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR203106 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGATCCTCCTGCTACTGCTGACCTTGTCTCTGGCCTCCAGGACAAAGGCAGGGGAGATCATCGGGG
  GACATGAAGTCAAGCCCCACTCTCGACCCTACATGGCCTTACTTTCGATCAAGGATCAGCAGCCTGAGGC
  GATATGTGGGGGCTTCCTTATTCGAGAGGACTTTGTGCTGACTGCTGCTCACTGTGAAGGAAGTATAATA
  AATGTCACTTTGGGGGCCCACAACATCAAAGAACAGGAGAAGACCCAGCAAGTCATCCCTATGGTAAAAT
  GCATTCCCCACCCAGACTATAATCCTAAGACATTCTCCAATGACATCATGCTGCTAAAGCTGAAGAGTAA
  GGCCAAGAGGACTAGAGCTGTGAGGCCCCTCAACCTGCCCAGGCGCAATGTCAATGTGAAGCCAGGAGAT
  GTGTGCTATGTGGCTGGTTGGGGAAGGATGGCCCCAATGGGCAAATACTCAAACACGCTACAAGAGGTTG
  AGCTGACAGTACAGAAGGATCGGGAGTGTGAGTCCTACTTTAAAAATCGTTACAACAAAACCAATCAGAT
  ATGTGCGGGGGACCCAAAGACCAAACGTGCTTCCTTTCGGGGGGATTCTGGAGGCCCGCTTGTGTGTAAA
  AAAGTGGCTGCAGGCATAGTTTCCTATGGATATAAGGATGGTTCACCTCCACGTGCTTTCACCAAAGTCT
  CGAGTTTCTTATCCTGGATAAAGAAAACAATGAAAAGCAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR203106 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKILLLLLTLSLASRTKAGEIIGGHEVKPHSRPYMALLSIKDQQPEAICGGFLIREDFVLTAAHCEGSII
  NVTLGAHNIKEQEKTQQVIPMVKCIPHPDYNPKTFSNDIMLLKLKSKAKRTRAVRPLNLPRRNVNVKPGD
  VCYVAGWGRMAPMGKYSNTLQEVELTVQKDRECESYFKNRYNKTNQICAGDPKTKRASFRGDSGGPLVCK
  KVAAGIVSYGYKDGSPPRAFTKVSSFLSWIKKTMKSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013542
• ORF Size: 744 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_013542.1, NM_013542.2, NM_013542.3, NP_038570.1
• RefSeq Size: 1418
• RefSeq ORF: 744
• Locus ID: 14939
• MW: 27.5 kDa
• Gene Summary: This gene encodes a member of the granzyme subfamily of proteins, part of the peptidase S1 family of serine proteases. The encoded preproprotein is secreted by natural killer (NK) cells and cytotoxic T lymphocytes (CTLs) and proteolytically processed to generate the active protease, which induces target cell apoptosis. This protein also processes cytokines and degrades extracellular matrix proteins, and these roles are implicated in chronic inflammation and wound healing. Mice lacking a functional copy of this gene exhibit impaired immune cell-mediated cytolysis. [provided by RefSeq, Sep 2016]