Mpdu1 (BC026776) Mouse Tagged ORF Clone

CAT#: MR203118

Mpdu1 (Myc-DDK-tagged) - Mouse mannose-P-dolichol utilization defect 1 (cDNA clone MGC:25609 IMAGE:4159355)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mpdu1
• Synonyms: SL15, LEC35
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR203118 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGGGGAGGCCGACGGGCCGTTCAAAGGGCTGCTGGTGCCAATTCTTTTACCTGAGAAATGTTACG
  ACCAGCTCTTCGTGCAATGGGACTTGCTTCATGTTCCCTGCCTCAAGATTCTCCTCAGCAAAGGCCTCGG
  GCTGGGCATCGTGGCTGGGTCACTTCTCGTCAAGCTGCCCCAGGTATTTAAACTCTTGGGAGCCAAGAGT
  GCAGAAGGACTGAGTCTCCAGTCAGTAATGCTGGAGCTAGTGGCACTGACCGGAACCGTGGTCTACAGCA
  TCACCAACAACTTCCCCTTCAGCTCTTGGGGTGAAGCGCTGTTCCTGACACTCCAGACCGTTGCCATCTG
  CTTCCTGGTCATGCACTACAGAGGAGAGACCGTGAAAGGAGTCGCTTTCCTTGCCTGCTATGCCATGGTC
  CTGCTGGCGCTGCTCTCCCCGCTCACGCCTCTGGCTGTAGTCACTCTGCTCCAGGCCTCCAATGTACCTG
  CCGTGGTGGTGGGGAAGTTGCTTCAGGCAGCCACTAACTACCGCAACGGACACACAGGCCAGCTTTCAGC
  CATTACAGTGTTTATGCTGTTTGGGGGCTCCTTGGCCCGAATCTTCACTTCTGTTCAGGAAACTGGAGAC
  CCCCTCATGGCTGGAGTCTTTGTGGTCTCTTCTCTCTGCAATGGCCTCATTGCTGCCCAGGTCCTCTTCT
  ACTGGAACGCAAAGGCTCCCCACAAACAGAAAAAGGAGCAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR203118 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAGEADGPFKGLLVPILLPEKCYDQLFVQWDLLHVPCLKILLSKGLGLGIVAGSLLVKLPQVFKLLGAKS
  AEGLSLQSVMLELVALTGTVVYSITNNFPFSSWGEALFLTLQTVAICFLVMHYRGETVKGVAFLACYAMV
  LLALLSPLTPLAVVTLLQASNVPAVVVGKLLQAATNYRNGHTGQLSAITVFMLFGGSLARIFTSVQETGD
  PLMAGVFVVSSLCNGLIAAQVLFYWNAKAPHKQKKEQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC026776
• ORF Size: 741 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC026776, AAH26776
• RefSeq Size: 1259 bp
• RefSeq ORF: 743 bp
• Locus ID: 24070
• MW: 26.4 kDa
• Gene Summary: This gene encodes a member of the PQ-loop superfamily. A similar gene in human encodes a protein that is required for monosaccharide-P-dolichol-dependent glycosyltransferase reactions, and disruption of this gene is the cause of congenital disorder of glycosylation (CDG) type 1F, a disease linked to defects in protein N-glycosylation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2014]