Usp12 (BC049274) Mouse Tagged ORF Clone

CAT#: MR203192

  • TrueORF®

Usp12 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 12 (cDNA clone MGC:55011 IMAGE:4500832)


  "BC049274" in other vectors (4)


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Reconstitution Protocol

USD 68.00

USD 670.00

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Size
    • 10 ug

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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Usp12
Synonyms Ubh1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR203192 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAACAAGATGCCCATGAATTCTTAAATTACCTACTCAATACAATTGCTGATATTCTACAAGAAGAAA
GAAAACAGGAGAAGCAAAATGGCCGATTACGGAACGGAGACGTGGACAACGAGGATAACAACAGCACACC
AGACCCAACCTGGGTCCACGAGATCTTTCAGGGAACATTAACTAATGAAACCAGGTGTCTTACTTGTGAA
ACTATAAGCAGCAAAGATGAAGATTTTTTAGACCTTTCTGTTGACGTGGAACAAAACACATCAATTACTC
ACTGCTTAAGAGGGTTCAGTAACACAGAAACTCTGTGCAGTGAATATAAGTACTACTGTGAAGAGTGCCG
GAGCAAGCAGGAAGCACACAAGCGGATGAAGGTTAAGAAACTGCCCCTGATCCTGGCTCTGCACCTGAAG
AGGTTTAAGTATATGGACCAGCTTCACCGGTACACGAAGCTTTCTTATCGCGTGGTCTTTCCTTTAGAGC
TCCGTCTGTTCAACACGTCAGGAGATGCGACCAACCCTGACAGAATGTATGACCTGGTCGCCGTTGTGGT
TCACTGTGGAAGTGGTCCCAACCGAGGCCATTATATTGCAATAGTTAAGAGTCATGATTTCTGGCTGTTG
TTTGATGATGACATTGTAGAAAAAATCGATGCCCAAGCTATTGAAGAATTCTACGGATTGACATCTGACA
TCTCCAAGAACTCGGAGTCTGGCTACATCCTTTTCTATCAGTCTCGGGAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR203192 protein sequence
Red=Cloning site Green=Tags(s)

MQQDAHEFLNYLLNTIADILQEERKQEKQNGRLRNGDVDNEDNNSTPDPTWVHEIFQGTLTNETRCLTCE
TISSKDEDFLDLSVDVEQNTSITHCLRGFSNTETLCSEYKYYCEECRSKQEAHKRMKVKKLPLILALHLK
RFKYMDQLHRYTKLSYRVVFPLELRLFNTSGDATNPDRMYDLVAVVVHCGSGPNRGHYIAIVKSHDFWLL
FDDDIVEKIDAQAIEEFYGLTSDISKNSESGYILFYQSRD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC049274
ORF Size 750 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq BC049274, AAH49274
RefSeq Size 3928 bp
RefSeq ORF 752 bp
Locus ID 22217
MW 29.2 kDa

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