H2 (BC002237) Mouse Tagged ORF Clone

CAT#: MR203394

H2 (Myc-DDK-tagged) - Mouse histocompatibility 2, class II, locus Mb1 (cDNA clone MGC:7514 IMAGE:3491702)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: H2
• Synonyms: AI385589|H-2Mb1|H2-Mb1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR203394 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCACTCTGGCTGCTGCTGCTGGTCCTCAGTCTGCACTGTATGGGGGCAGGTGGCTTTGTGGCTC
  ATGTGGAAAGCACGTGCGTGCTGGATGATGCTGGAACCCCACAGGACTTCACATACTGTGTTTCCTTCAA
  CAAAGATCTGCTGGCCTGCTGGGATCCAATAGTGGGAAAAATAGTCCCCTGTGAATTTGGGGTGCTGTAT
  CCATTGGCCGAAAATTTTTCAAGGATCCTCAACAAAGAAGAGAGTCTTCTCCAGCGTTTGCAAAACGGGC
  TTCCGGACTGTGCCTCCCACACCCAGCCCTTCTGGAATGCGCTGACCCACAGAACGAGACCGCCATCTGT
  CCGAGTAGCCCAAACCACACCTTTTAACACAAGGGAGCCCGTGATGCTGGCCTGCTACGTCTGGGGCTTC
  TATCCAGCGGATGTGACCATCACATGGATGAAGAATGGGCAGCTTGTCCCTTCCCACAGCAACAAGGAGA
  AGACGGCTCAGCCCAATGGAGACTGGACATACCAGACAGTCTCCTACCTAGCCCTAACCCCTTCCTACGG
  GGACGTCTACACCTGCGTGGTTCAGCACAGCGGGACCTCTGAGCCCATCCGCGGGGACTGGACACCTGGG
  CTGTCCCCCATCCAGACAGTGAAGGTCTCTGTGTCTGCAGCCACCCTGGGCCTGGGCTTCACCATCTTCT
  GTGTTGGCTTCTTCAGATGGCGCAAGTCTCATTCCTCCAGCTACACTCCTCTCTCTGGATCCACCTACCC
  AGAAGGACGGCAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR203394 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAALWLLLLVLSLHCMGAGGFVAHVESTCVLDDAGTPQDFTYCVSFNKDLLACWDPIVGKIVPCEFGVLY
  PLAENFSRILNKEESLLQRLQNGLPDCASHTQPFWNALTHRTRPPSVRVAQTTPFNTREPVMLACYVWGF
  YPADVTITWMKNGQLVPSHSNKEKTAQPNGDWTYQTVSYLALTPSYGDVYTCVVQHSGTSEPIRGDWTPG
  LSPIQTVKVSVSAATLGLGFTIFCVGFFRWRKSHSSSYTPLSGSTYPEGRH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC002237
• ORF Size: 783 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC002237, AAH02237
• RefSeq Size: 1282 bp
• RefSeq ORF: 785 bp
• Locus ID: 14999
• MW: 28.9 kDa