H2 (BC010322) Mouse Tagged ORF Clone

CAT#: MR203516

H2 (Myc-DDK-tagged) - Mouse histocompatibility 2, class II antigen A, beta 1 (cDNA clone MGC:6297 IMAGE:2651058)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: H2
• Synonyms: Abeta, IAb, I-A, I-Abeta, Rmcs1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR203516 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCTGCAGATCCCCAGCCTCCTCCTCTCGGCTGCTGTGGTGGTGCTGATGGTGCTGAGCAGCCCAG
  GGACTGAGGGCGGAGACTCCGAAAGGCATTTCGTGGTCCAGTTCAAGGGCGAGTGCTACTACACCAACGG
  GACGCAGCGCATACGGCTCGTGACCAGATACATCTACAACCGGGAGGAGTACGTGCGCTACGACAGCGAC
  GTGGGCGAGTACCGCGCGGTGACCGAGCTGGGGCGGCCAGACGCCGAGTACTGGAACAGCCAGCCGGAGA
  TCCTGGAGCGAACGCGGGCCGAGGTGGACACGGCGTGCAGACACAACTACGAGGAGACGGAGGTCCCCAC
  CTCCCTGCGGCGGCTTGAACAGCCCAATGTCGCCATCTCCCTGTCCAGGACAGAGGCCCTCAACCACCAC
  AACACTCTGGTCTGTTCGGTGACAGATTTCTACCCAGCCAAGATCAAAGTGCGCTGGTTCAGGAATGGCC
  AGGAGGAGACAGTGGGGGTCTCATCCACACAGCTTATTAGGAATGGGGACTGGACCTTCCAGGTCCTGGT
  CATGCTGGAGATGACCCCTCATCAGGGAGAGGTCTACACCTGCCATGTGGAGCATCCCAGCCTGAAGAGC
  CCCATCACTGTGGAGTGGAGGGCACAGTCCGAGTCTGCCCGGAGCAAGATGTTGAGCGGCATCGGGGGCT
  GCGTGCTTGGGGTGATCTTCCTCGGGCTCGGCCTTTTCATCCGTCACAGGAGTCAGAAAGGACCTCGAGG
  CCCTCCTCCAGCAGGGCTCCTGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR203516 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MALQIPSLLLSAAVVVLMVLSSPGTEGGDSERHFVVQFKGECYYTNGTQRIRLVTRYIYNREEYVRYDSD
  VGEYRAVTELGRPDAEYWNSQPEILERTRAEVDTACRHNYEETEVPTSLRRLEQPNVAISLSRTEALNHH
  NTLVCSVTDFYPAKIKVRWFRNGQEETVGVSSTQLIRNGDWTFQVLVMLEMTPHQGEVYTCHVEHPSLKS
  PITVEWRAQSESARSKMLSGIGGCVLGVIFLGLGLFIRHRSQKGPRGPPPAGLLQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC010322
• ORF Size: 795 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC010322, AAH10322
• RefSeq Size: 1251 bp
• RefSeq ORF: 797 bp
• Locus ID: 14961
• MW: 29.9 kDa