Rpl7a (BC065176) Mouse Tagged ORF Clone

CAT#: MR203523

Rpl7a (Myc-DDK-tagged) - Mouse ribosomal protein L7a (cDNA clone MGC:63353 IMAGE:6819288)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rpl7a
• Synonyms: Surf3, Surf-3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR203523 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCAAAGGGAAGAAGGCCAAGGGGAAGAAGGTGGCCCCGGCCCCCGCCGTCGTCAAGAAGCAGGAGG
  CCAAGAAGGTGGTCAACCCTCTGTTCGAGAAGAGGCCCAAGAACTTCGGCATTGGACAGGACATCCAGCC
  CAAAAGAGATTTAACGCGCTTCTTCAAATGGCCCCGCTACATCAGACTGCAGCGGCAAAGAGCTATCCTC
  TACAAGCGGCTCAAAGTCCCTCCTGCCATTAACCAGTTCACCCAGGCCCTGGACAGGCAGACAGCTACTC
  AGCTGCTTAAGCTTGCCCACAAGTACAGGCCAGAGACAAAGCAAGAGAAGAAGCAAAGGCTACTGGCCCG
  TGCTGAGAAGAAAGCTGCTGGCAAAGGCGACGTCCCAACTAAGAGACCACCTGTCCTCCGAGCAGGAGTC
  AATACAGTCACCACCTTGGTGGAGAACAAGAAGGCTCAGCTGGTGGTGATTGCCCATGACGTAGACCCCA
  TTGAGCTGGTGGTTTTCCTACCTGCTCTGTGTCGAAAGATGGGGGTGCCCTACTGCATCATCAAGGGAAA
  GGCCAGGCTGGGGCACCTGGTCCACAGGAAGACATGCACCACCGTTGCCTTCACACAGGTTAACTCGGAA
  GACAAGGGTGCTCTGGCTAAGCTGGTGGAAGCTATTAGGACCAATTATAATGACAGATATGACGAGATCC
  GTCGCCACTGGGGAGGCAACGTCCTGGGTCCTAAGTCTGTGGCTCGAATTGCCAAGCTGGAAAAAGCAAA
  GGCTAAAGAACTCGCCACTAAATTGGGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR203523 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPKGKKAKGKKVAPAPAVVKKQEAKKVVNPLFEKRPKNFGIGQDIQPKRDLTRFFKWPRYIRLQRQRAIL
  YKRLKVPPAINQFTQALDRQTATQLLKLAHKYRPETKQEKKQRLLARAEKKAAGKGDVPTKRPPVLRAGV
  NTVTTLVENKKAQLVVIAHDVDPIELVVFLPALCRKMGVPYCIIKGKARLGHLVHRKTCTTVAFTQVNSE
  DKGALAKLVEAIRTNYNDRYDEIRRHWGGNVLGPKSVARIAKLEKAKAKELATKLG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC065176
• ORF Size: 798 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC065176, AAH65176
• RefSeq Size: 891 bp
• RefSeq ORF: 800 bp
• Locus ID: 27176
• MW: 32.7 kDa