Phf7 (BC057174) Mouse Tagged ORF Clone

CAT#: MR203559

Phf7 (Myc-DDK-tagged) - Mouse PHD finger protein 7 (cDNA clone MGC:68075 IMAGE:5004450)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Phf7
• Synonyms: 1700006H01Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR203559 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGACTTTAAAAGAAAAAAACAAACATCCAAGATTGAGAAAAACTATCAGGACTAAGAAGGTAACAC
  AGAGGAAGCTGTCTTCAAGTCCTGTTTGCCTTCTATGCCTTCAAGAACCTGGTGATCCTGAAAAACTAGG
  AGAATTTCTTCAGAAAGACAATCTTTGTGTCCATTATTTCTGTCTTATCCTGTCTAGCAGACTGCCTCAG
  AAGGGCCAACCCAACAGAGGTCTCCATGGATTTATGCCTGAAGACATCAAAAGGGAGGCAGTACGGGCTT
  CTAAGAAGATCTGCTTTGTATGCAAGAAAAAAGGAGCTGCTATCAGATGCCAGAATGATCAGTGTGTCCA
  GAACTTCCATCTGCCTTGTGGCCAAGAAAGGGGTTGCCTTTCACAGTTTTTTGGAGAGTACAAATCATAT
  TGTAGAAAACATCGACCAACACAGAACATCCACCAGGGGAGTTTGGGGGAGGAAAGCTGTGTCTTGTGTT
  GCGAAAATTTATCCCGAACAAGTGTTGAGAACATCCAGAGCCCATGCTGTAGTCAAGCTATCTATCACCG
  CAAGTGTATCCAGAAATATGCCCACACATCAGCAAAGCACTTCTTCAAGTGTCCCCAGTGCAACAACCGA
  GAAGAGTTCCCACAGGAGATGCTGAGGATGGGGATCCACATCCCAGACAGACGGTGGCGCCTCATTTTGT
  GTGCTACGTGTGGATCCCATGGAACCCACCGGGACTGCTCCTCTCTTAGACCAAACAGCAAGAAATGGGA
  ATGTAATGAGTGTTTGCCTGCTTCAACCACGAGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR203559 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKTLKEKNKHPRLRKTIRTKKVTQRKLSSSPVCLLCLQEPGDPEKLGEFLQKDNLCVHYFCLILSSRLPQ
  KGQPNRGLHGFMPEDIKREAVRASKKICFVCKKKGAAIRCQNDQCVQNFHLPCGQERGCLSQFFGEYKSY
  CRKHRPTQNIHQGSLGEESCVLCCENLSRTSVENIQSPCCSQAIYHRKCIQKYAHTSAKHFFKCPQCNNR
  EEFPQEMLRMGIHIPDRRWRLILCATCGSHGTHRDCSSLRPNSKKWECNECLPASTTS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC057174
• ORF Size: 804 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC057174, AAH57174
• RefSeq Size: 1768 bp
• RefSeq ORF: 806 bp
• Locus ID: 71838
• MW: 30.8 kDa
• Gene Summary: May play a role in spermatogenesis. [UniProtKB/Swiss-Prot Function]