Pitpnb (BC034676) Mouse Tagged ORF Clone

CAT#: MR203641

Pitpnb (Myc-DDK-tagged) - Mouse phosphatidylinositol transfer protein, beta (cDNA clone MGC:25322 IMAGE:4481685)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pitpnb
• Synonyms: PI-TP-beta
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR203641 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCTGATTAAGGAATTCCGTGTGGTTTTGCCGTGTTCTGTTCAAGAGTATCAGGTTGGACAGCTTT
  ACTCTGTTGCAGAAGCGAGTAAGAATGAAACTGGTGGTGGAGAAGGAATCGAAGTCCTGAAGAATGAACC
  TTATGAAAACGATGGCGAGAAGGGACAGTACACACACAAAATCTACCACCTGAAGAGCAAAGTTCCTGCA
  TTTGTGAGGATGATTGCTCCCGAGGGCTCCCTGGTGTTTCATGAGAAAGCCTGGAATGCCTACCCCTACT
  GCAGAACAATTGTAACGAATGAATACATGAAAGATGACTTCTTCATCAAAATTGAAACATGGCATAAACC
  TGACTTGGGAACATTAGAAAATGTTCACGGTTTAGATCCCAACACTTGGAAAACTGTTGAAATTGTCCAC
  ATAGACATTGCAGATCGAAGTCAAGTTGAACCAGCAGACTACAAAGCTGATGAAGACCCTGCATTATTCC
  ATTCAGTCAAGACCAAGAGAGGACCCCTGGGACCTAACTGGAAGAAGGAGCTGGCAAACACCCCTGACTG
  TCCTAGGATGTGTGCCTATAAGCTGGTGACCATCAAGTTCAAGTGGTGGGGGCTGCAGAGCAAAGTAGAG
  AACTTTATCCAGAAGCAAGAAAAACGGATATTTACGAACTTACATCGCCAGCTCTTTTGTTGGATTGACA
  AGTGGATTGACCTGACAATGGAAGACATTAGGCGAATGGAGGATGAGACTCAGAAAGAACTAGAAACACT
  ACGGAGTCAAGGCCAAGTGAGGGGGACCAGTGCGGCCTGTGATGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR203641 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVLIKEFRVVLPCSVQEYQVGQLYSVAEASKNETGGGEGIEVLKNEPYENDGEKGQYTHKIYHLKSKVPA
  FVRMIAPEGSLVFHEKAWNAYPYCRTIVTNEYMKDDFFIKIETWHKPDLGTLENVHGLDPNTWKTVEIVH
  IDIADRSQVEPADYKADEDPALFHSVKTKRGPLGPNWKKELANTPDCPRMCAYKLVTIKFKWWGLQSKVE
  NFIQKQEKRIFTNLHRQLFCWIDKWIDLTMEDIRRMEDETQKELETLRSQGQVRGTSAACDD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC034676
• ORF Size: 816 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC034676, AAH34676
• RefSeq Size: 2681 bp
• RefSeq ORF: 818 bp
• Locus ID: 56305
• MW: 31.6 kDa
• Gene Summary: This gene encodes a member of the phosphatidylinositol transfer protein family. The encoded protein catalyzes the transfer of phospholipids (phosphatidylinositol and phosphatidylcholine) between membranes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2014]