Ppp2cb (BC019161) Mouse Tagged ORF Clone

CAT#: MR203742

Ppp2cb (Myc-DDK-tagged) - Mouse protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform (cDNA clone MGC:29430 IMAGE:3674696)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ppp2cb
• Synonyms: PP2Ac
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR203742 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGACAAGGCGTTCACCAAGGAGCTGGACCAGTGGGTGGAGCAGCTGAACGAGTGTAAGCAGCTGA
  ACGAGAACCAAGTGCGGACGCTGTGCGAGAAGGCTAAGGAAATTTTAACGAAAGAATCAAATGTGCAAGA
  GGTCCGCTGTCCTGTTACCGTCTGTGGAGATGTGCATGGCCAATTCCATGACCTTATGGAGCTCTTCAGA
  ATTGGTGGGAAATCACCAGATACGAACTACCTATTCATGGGGGACTATGTAGACAGAGGCTACTACTCTG
  TGGAGACTGTGACTCTTCTTGTAGCGTTAAAGGTGCGCTATCCAGAGCGCATCACAATATTGCGAGGAAA
  TCACGAAAGCCGGCAGATCACACAAGTGTATGGCTTTTATGATGAGTGCCTACGGAAGTATGGAAATGCC
  AACGTGTGGAAATACTTTACAGATCTCTTTGATTATCTTCCACTTACAGCTTTAGTAGATGGACAGATAT
  TCTGCCTCCACGGTGGCCTGTCTCCATCCATAGATACACTGGACCACATTTCTCCACGTGGTGCTGGCTA
  CACATTTGGACAAGACATTTCTGAAACATTTAACCATGCCAACGGTCTCACACTGGTGTCTCGTGCTCAC
  CAGCTTGTAATGGAAGGATATAACTGGTGCCATGATCGGAATGTGGTCACCATTTTTAGTGCGCCCAATT
  ACTGCTACCGTTGTGGGAACCAGGCTGCTATCATGGAATTAGATGACACTTTAAAATATTCATTTCTTCA
  GTTTGACCCAGCACCTCGTCGTGGAGAGCCTCATGTGACCCGGCGCACCCCAGACTACTTCCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR203742 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDDKAFTKELDQWVEQLNECKQLNENQVRTLCEKAKEILTKESNVQEVRCPVTVCGDVHGQFHDLMELFR
  IGGKSPDTNYLFMGDYVDRGYYSVETVTLLVALKVRYPERITILRGNHESRQITQVYGFYDECLRKYGNA
  NVWKYFTDLFDYLPLTALVDGQIFCLHGGLSPSIDTLDHISPRGAGYTFGQDISETFNHANGLTLVSRAH
  QLVMEGYNWCHDRNVVTIFSAPNYCYRCGNQAAIMELDDTLKYSFLQFDPAPRRGEPHVTRRTPDYFL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC019161
• ORF Size: 834 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC019161, AAH19161
• RefSeq Size: 1532 bp
• RefSeq ORF: 836 bp
• Locus ID: 19053
• MW: 32.1 kDa
• Gene Summary: PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. [UniProtKB/Swiss-Prot Function]