Fhl1 (BC031120) Mouse Tagged ORF Clone

CAT#: MR203785

Fhl1 (Myc-DDK-tagged) - Mouse four and a half LIM domains 1 (cDNA clone MGC:36075 IMAGE:4989977)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Fhl1
• Synonyms: KyoT
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR203785 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGAGAAGTTCGACTGTCACTACTGCAGGGACCCCTTGCAGGGGAAGAAGTACGTGCAGAAGGATG
  GCCGTCACTGCTGCCTGAAGTGCTTTGACAAGTTCTGCGCCAACACCTGCGTGGACTGCCGCAAGCCCAT
  AAGCGCTGATGCCAAGGAGGTGCATTATAAGAATCGCTACTGGCACGACAACTGCTTCCGCTGTGCCAAG
  TGCCTTCACCCCTTGGCCAGTGAGACCTTTGTGTCCAAGGATGGCAAGATCCTGTGCAACAAGTGCGCTA
  CTCGGGAGGACTCCCCCAGGTGCAAAGGGTGCTTCAAGGCCATTGTGGCAGGAGACCAGAACGTGGAGTA
  CAAGGGCACCGTCTGGCATAAAGACTGCTTCACCTGCAGCAACTGCAAGCAAGTCATTGGGACCGGAAGC
  TTCTTCCCGAAAGGGGAGGACTTCTACTGTGTGACTTGCCATGAGACCAAGTTCGCCAAACATTGCGTGA
  AGTGCAACAAGGCCATCACATCTGGAGGAATCACTTACCAGGATCAGCCCTGGCATGCCGAGTGCTTTGT
  GTGTGTTACCTGCTCTAAGAAGCTGGCTGGGCAGCGTTTCACCGCTGTGGAGGACCAGTATTACTGCGTG
  GATTGCTACAAGAACTTTGTGGCCAAGAAGTGTGCTGGATGCAAGAACCCCATCACTGGGTTTGGTAAAG
  GCTCCAGTGTGGTGGCCTATGAAGGACAATCCTGGCACGACTACTGCTTCCACTGCAAAAAATGCTCCGT
  GAATCTGGCCAACAAGCGCTTTGTATTTCATAATGAGCAGGTGTATTGCCCTGACTGTGCCAAAAAGCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR203785 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSEKFDCHYCRDPLQGKKYVQKDGRHCCLKCFDKFCANTCVDCRKPISADAKEVHYKNRYWHDNCFRCAK
  CLHPLASETFVSKDGKILCNKCATREDSPRCKGCFKAIVAGDQNVEYKGTVWHKDCFTCSNCKQVIGTGS
  FFPKGEDFYCVTCHETKFAKHCVKCNKAITSGGITYQDQPWHAECFVCVTCSKKLAGQRFTAVEDQYYCV
  DCYKNFVAKKCAGCKNPITGFGKGSSVVAYEGQSWHDYCFHCKKCSVNLANKRFVFHNEQVYCPDCAKKL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC031120
• ORF Size: 840 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC031120, AAH31120
• RefSeq Size: 2245
• RefSeq ORF: 842
• Locus ID: 14199
• MW: 31.9 kDa
• Gene Summary: May have an involvement in muscle development or hypertrophy. Isoform 2 binds to RBP-J and plays a negative regulatory role in the RBP-J-mediated transcription in mammalian systems. [UniProtKB/Swiss-Prot Function]