Lix1 (BC049574) Mouse Tagged ORF Clone

CAT#: MR203821

Lix1 (Myc-DDK-tagged) - Mouse limb expression 1 homolog (chicken) (cDNA clone MGC:58187 IMAGE:6593108)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Lix1
• Synonyms: 5730466L18Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR203821 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACAGAACCTTGGAATCTCTGAGGCACATCATTGCCCAAGCCTTGCCTCACAGAGACCCGGCTTTGG
  TCTTCAAAGACTTGAATGTTGTGTCAATGTTACAGGAATTTTGGGAAAGCAAGCAGCAGCAGAAAGCCAC
  CTTCTCCAGTGAGGGACTGGTGGTCTATGAGTCAATGCCTTCCTCTGGGCCTCCCTTTGTGAGCTATGTG
  ACTCTCCCTGGAGGGAGCTGCTTTGGCAACTTTCAGTGCTGCTTAAGTAGAGCTGAGGCCAGGCGGGATG
  CTGCTAAAGTGGCGCTCATCAACTCTCTCTTCAATGAGCTACCCTCCCGAAGGATCACCAAGGAGTTCAT
  CATGGAGAGTGTTCAGGAAGCAGTAGCCTCCACCAGGGGCACGTTAGATGATGCGGATGACCCAAGCACA
  AGTGTGGGAGCCTATCACTACATGCTGGAGTCAAACATGGGGAAGACTATGCTGGAGTTTCAGGAACTAA
  TGACCATTTTCCAACTACTGCACTGGAATGGAAGCCTGAAAGCCCTCCGTGAAACAAAGTGCTCTCGACA
  GGAAGTCATCTCCTACTATTCCCAATATTCTTTAGATGAAAAGATGCGTAGCCACATGGCCCTGGACTGG
  ATTATGAAGGAGCGTGAATCACCAGGAATTCTCTCTCAAGAGCTGCGTGCAGCCCTAGGGCAGCTGGAGG
  AAGCCAGGAAGGCTGGACAAGAACTACGATTTTACAAAGAAAAAAAAGAAATCCTGAGCTTGGCCCTGAC
  TCAGATCTATAGTGACCCTGACCCTTCCTCACCCAGTGATGACCAGCTAAGCCTCACGGCTCTGTGTGGC
  TATCAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR203821 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDRTLESLRHIIAQALPHRDPALVFKDLNVVSMLQEFWESKQQQKATFSSEGLVVYESMPSSGPPFVSYV
  TLPGGSCFGNFQCCLSRAEARRDAAKVALINSLFNELPSRRITKEFIMESVQEAVASTRGTLDDADDPST
  SVGAYHYMLESNMGKTMLEFQELMTIFQLLHWNGSLKALRETKCSRQEVISYYSQYSLDEKMRSHMALDW
  IMKERESPGILSQELRAALGQLEEARKAGQELRFYKEKKEILSLALTQIYSDPDPSSPSDDQLSLTALCG
  YH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC049574
• ORF Size: 846 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC049574.1
• RefSeq Size: 1367 bp
• RefSeq ORF: 848 bp
• Locus ID: 66643
• MW: 31.9 kDa