Tia1 (BC023813) Mouse Tagged ORF Clone

CAT#: MR203884

  • TrueORF®

Tia1 (Myc-DDK-tagged) - Mouse cytotoxic granule-associated RNA binding protein 1 (cDNA clone MGC:36034 IMAGE:5353938)


  "BC023813" in other vectors (4)


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Reconstitution Protocol

USD 68.00

USD 670.00

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Size
    • 10 ug

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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Tia1
Synonyms mTIA-1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR203884 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGACGAGATGCCCAAGACTCTATACGTCGGTAACCTTTCTCGAGATGTGACAGAAGCTCTCATCC
TCCAGCTCTTTAGCCAGATTGGACCTTGTAAAAACTGCAAAATGATTATGGATACAGCAGGAAATGACCC
ATACTGTTTTGTGGAGTTCCATGAGCACCGTCATGCAGCTGCAGCCCTGGCTGCCATGAATGGGCGGAAG
ATAATGGGTAAGGAAGTGAAAGTGAATTGGGCAACAACCCCTAGCAGTCAAAAGAAGGATACGAGCAATC
ATTTCCATGTGTTTGTTGGTGACCTCAGTCCAGAAATCACAACCGAAGACATCAAAGCAGCGTTTGCACC
ATTTGGAAGAATTTCAGATGCCCGTGTGGTAAAAGACATGGCTACCGGGAAGTCTAAGGGATATGGCTTT
GTCTCCTTTTTCAACAAATGGGATGCAGAAAATGCCATTCAGCAGATGGGTGGCCAGTGGCTTGGTGGAA
GACAAATCAGAACTAACTGGGCAACCCGAAAGCCTCCAGCTCCAAAGAGTACATATGAGTCGAACACCAA
ACAGCTGTCATATGATGAGGTTGTGAGTCAGTCTAGTCCCAACAACTGCACTGTGTACTGTGGAGGGGTG
ACGTCAGGACTGACAGAACAACTAATGCGTCAGACTTTTTCTCCATTTGGACAAATAATGGAAATTCGAG
TCTTCCCAGATAAAGGATACTCATTTGTTCGTCAGACCCAGTCTTCTGCACCGTGGATGGGACCCAATTA
CAGTGTGCCACCACCTCAAGGGCAGAATGGCAGCATGTTGCCTAGTCAGCCTGCTGGGTATCGAGTGGCC
GGGTATGAAACCCAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR203884 protein sequence
Red=Cloning site Green=Tags(s)

MEDEMPKTLYVGNLSRDVTEALILQLFSQIGPCKNCKMIMDTAGNDPYCFVEFHEHRHAAAALAAMNGRK
IMGKEVKVNWATTPSSQKKDTSNHFHVFVGDLSPEITTEDIKAAFAPFGRISDARVVKDMATGKSKGYGF
VSFFNKWDAENAIQQMGGQWLGGRQIRTNWATRKPPAPKSTYESNTKQLSYDEVVSQSSPNNCTVYCGGV
TSGLTEQLMRQTFSPFGQIMEIRVFPDKGYSFVRQTQSSAPWMGPNYSVPPPQGQNGSMLPSQPAGYRVA
GYETQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC023813
ORF Size 855 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq BC023813, AAH23813
RefSeq Size 1455 bp
RefSeq ORF 857 bp
Locus ID 21841
MW 31.6 kDa

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