Otx2 (NM_144841) Mouse Tagged ORF Clone
CAT#: MR203937
- TrueORF®
Otx2 (Myc-DDK-tagged) - Mouse orthodenticle homolog 2 (Drosophila) (Otx2)
"NM_144841" in other vectors (6)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Otx2 |
Synonyms | E130306E05Rik |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR203937 representing NM_144841
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGTCTTATCTAAAGCAACCGCCTTACGCAGTCAATGGGCTGAGTCTGACCACTTCGGGTATGGACT TGCTGCATCCCTCCGTGGGCTACCCCGCCACCCCCCGGAAACAGCGAAGGGAGAGGACGACATTTACTAG GGCACAGCTCGACGTTCTGGAAGCTCTGTTTGCCAAGACCCGGTACCCAGACATCTTCATGAGGGAAGAG GTGGCACTGAAAATCAACTTGCCAGAATCCAGGGTGCAGGTATGGTTTAAGAATCGAAGAGCTAAGTGCC GCCAACAGCAGCAGCAGCAGCAGAATGGAGGTCAGAACAAAGTGAGGCCTGCCAAGAAGAAGAGCTCTCC AGCTCGGGAAGTGAGTTCAGAGAGTGGAACAAGTGGCCAGTTCAGTCCCCCCTCTAGTACCTCAGTCCCA ACCATTGCCAGCAGCAGTGCTCCAGTGTCTATCTGGAGCCCAGCGTCCATCTCCCCACTGTCTGACCCCT TGTCCACTTCCTCCTCCTGCATGCAGAGGTCCTATCCCATGACCTATACTCAGGCTTCAGGTTATAGTCA AGGCTATGCTGGCTCAACTTCCTACTTTGGGGGCATGGACTGTGGATCTTATTTGACCCCTATGCATCAC CAGCTTCCTGGACCAGGGGCCACACTCAGTCCCATGGGTACCAATGCTGTTACCAGCCATCTCAATCAGT CCCCAGCTTCTCTTTCCACCCAGGGATATGGAGCTTCAAGCTTGGGTTTTAACTCAACCACTGATTGCTT GGATTATAAGGACCAAACTGCCTCTTGGAAGCTTAACTTCAATGCTGACTGCTTGGATTATAAAGATCAG ACGTCCTCATGGAAATTCCAGGTTTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR203937 representing NM_144841
Red=Cloning site Green=Tags(s) MMSYLKQPPYAVNGLSLTTSGMDLLHPSVGYPATPRKQRRERTTFTRAQLDVLEALFAKTRYPDIFMREE VALKINLPESRVQVWFKNRRAKCRQQQQQQQNGGQNKVRPAKKKSSPAREVSSESGTSGQFSPPSSTSVP TIASSSAPVSIWSPASISPLSDPLSTSSSCMQRSYPMTYTQASGYSQGYAGSTSYFGGMDCGSYLTPMHH QLPGPGATLSPMGTNAVTSHLNQSPASLSTQGYGASSLGFNSTTDCLDYKDQTASWKLNFNADCLDYKDQ TSSWKFQVL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_144841 |
ORF Size | 867 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_144841.1, NM_144841.2, NM_144841.3, NM_144841.4, NP_659090.1 |
RefSeq Size | 2144 bp |
RefSeq ORF | 870 bp |
Locus ID | 18424 |
MW | 32.1 kDa |
Gene Summary | This gene encodes a protein that belongs to the homeobox family of transcription factors. The encoded protein plays a role in the development and patterning of the head. This protein regulates development of the choroid plexuses in the brain affecting composition of cerebrospinal fluid in the developing brain and is thought to function in the development of sense organs in the embryo. In humans, mutations in this gene are associated with pituitary hormone deficiency. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2013] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC207342 | Otx2 (untagged) - Mouse orthodenticle homolog 2 (Drosophila) (Otx2), (10ug) |
USD 420.00 |
|
MG203937 | Otx2 (GFP-tagged) - Mouse orthodenticle homolog 2 (Drosophila) (Otx2) |
USD 330.00 |
|
MR203937L1 | Lenti ORF clone of Otx2 (Myc-DDK-tagged) - Mouse orthodenticle homolog 2 (Drosophila) (Otx2) |
USD 500.00 |
|
MR203937L2 | Lenti ORF clone of Otx2 (mGFP-tagged) - Mouse orthodenticle homolog 2 (Drosophila) (Otx2) |
USD 624.00 |
|
MR203937L3 | Lenti ORF clone of Otx2 (Myc-DDK-tagged) - Mouse orthodenticle homolog 2 (Drosophila) (Otx2) |
USD 500.00 |
|
MR203937L4 | Lenti ORF clone of Otx2 (mGFP-tagged) - Mouse orthodenticle homolog 2 (Drosophila) (Otx2) |
USD 500.00 |
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