Pnp (BC052679) Mouse Tagged ORF Clone

CAT#: MR203940

Pnp (Myc-DDK-tagged) - Mouse purine-nucleoside phosphorylase (cDNA clone MGC:60531 IMAGE:30062908)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pnp
• Synonyms: AL024301|AU015798|Np|Np-1|Np-2|Pnp1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR203940 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAACGAGTTCACATACGAAGATTATGAGACCACTGCCAAGTGGCTTCTGCAACACACTGAATATC
  GACCTCAAGTGGCAGTGATCTGTGGTTCCGGCTTAGGAGGGCTGACTGCTCACTTAAAGGAGGCTCAGAT
  CTTTGACTACAATGAGATACCCAACTTTCCCCAAAGCACAGTGCAAGGTCACGCAGGCCGACTGGTGTTT
  GGATTGCTGAATGGCAGATGCTGTGTGATGATGCAAGGCCGGTTCCATATGTATGAAGGATACTCACTGT
  CAAAGGTGACATTCCCAGTGAGAGTTTTCCATCTTCTGGGTGTGGAAACTTTGGTGGTCACCAATGCTGC
  TGGAGGACTCAACCCTAATTTTGAAGTTGGAGATATTATGCTGATCCGTGATCACATCAACCTACCTGGT
  TTCTGTGGCCAGAACCCTCTCCGGGGCCCCAACGATGAAAGGTTTGGAGTTCGTTTTCCTGCCATGTCTG
  ATGCTTATGACCGGGATATGAGGCAGAAGGCTTTCACTGCCTGGAAACAAATGGGGGAGCAACGAAAGCT
  ACAAGAAGGCACCTACGTGATGTTGGCAGGCCCCAACTTTGAGACTGTGGCAGAGAGTCGTCTGCTAAAG
  ATGCTGGGAGCAGATGCTGTTGGCATGAGCACAGTCCCAGAAGTTATCGTCGCAAGGCACTGTGGGCTTC
  GAGTCTTTGGTTTCTCACTCATTACGAACAAGGTTGTCATGGATTATGAGAACTTGGAGAAGGCCAATCA
  CATGGAAGTTCTGGATGCCGGGAAAGCAGCTGCACAGACATTGGAAAGGTTTGTCTCCATTCTTATGGAG
  AGCATTCCACTCCCGGATCGTGGCAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR203940 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MENEFTYEDYETTAKWLLQHTEYRPQVAVICGSGLGGLTAHLKEAQIFDYNEIPNFPQSTVQGHAGRLVF
  GLLNGRCCVMMQGRFHMYEGYSLSKVTFPVRVFHLLGVETLVVTNAAGGLNPNFEVGDIMLIRDHINLPG
  FCGQNPLRGPNDERFGVRFPAMSDAYDRDMRQKAFTAWKQMGEQRKLQEGTYVMLAGPNFETVAESRLLK
  MLGADAVGMSTVPEVIVARHCGLRVFGFSLITNKVVMDYENLEKANHMEVLDAGKAAAQTLERFVSILME
  SIPLPDRGS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC052679
• ORF Size: 867 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC052679, AAH52679
• RefSeq Size: 1305 bp
• RefSeq ORF: 869 bp
• Locus ID: 18950
• MW: 32.3 kDa