Echs1 (NM_053119) Mouse Tagged ORF Clone

CAT#: MR203962

Echs1 (Myc-DDK-tagged) - Mouse enoyl Coenzyme A hydratase, short chain, 1, mitochondrial (Echs1), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Echs1
• Synonyms: C80529; SCEH
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR203962 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCCTGCGTGCTCTGCTGCCCAGAGCCTGTAGCTCACTGTTGTCCTCAGTCCGCTGCCCAGAAC
  TACGGCGCTTCGCCTCGGGTGCTAACTTTCAGTACATCATCACAGAAAAGAAAGGAAAGAATAGCAGCGT
  GGGGCTGATCCAGTTGAACCGCCCCAAAGCACTCAATGCACTTTGCAATGGCCTGATTGAGGAGCTCAAC
  CAAGCACTGGAGACCTTTGAGCAAGATCCTGCTGTGGGTGCCATTGTGCTCACTGGTGGGGATAAGGCCT
  TTGCAGCTGGAGCTGACATCAAGGAAATGCAGAACCGAACATTTCAGGACTGTTACTCCAGCAAGTTCCT
  GAGCCACTGGGACCACATCACCCGGGTCAAGAAACCGGTCATCGCAGCTGTCAATGGTTATGCTCTTGGT
  GGGGGTTGTGAACTTGCCATGATGTGTGATATCATCTATGCTGGCGAGAAAGCCCAGTTCGGACAGCCAG
  AAATCCTCCTGGGGACCATCCCACGTGCTGGAGGCACTCAGAGACTCACCCGAGCAGTCGGCAAATCGCT
  AGCAATGGAGATGGTCCTCACTGGTGACCGCATCTCAGCTCAGGATGCAAAGCAGGCAGGTCTTGTAAGC
  AAGATTTTTCCTGTTGAAAAACTGGTTGAAGAAGCCATCCAATGTGCAGAAAAAATTGCCAGCAATTCTA
  AAATCGTAGTAGCCATGGCGAAAGAATCTGTGAATGCAGCCTTTGAGATGACGTTAACAGAAGGAAATAA
  GCTGGAGAAGAGGCTTTTCTATTCCACCTTTGCCACCGATGACCGGAGAGAAGGGATGACTGCATTTGTA
  GAGAAAAGGAAGGCCAACTTCAAAGACCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR203962 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAALRALLPRACSSLLSSVRCPELRRFASGANFQYIITEKKGKNSSVGLIQLNRPKALNALCNGLIEELN
  QALETFEQDPAVGAIVLTGGDKAFAAGADIKEMQNRTFQDCYSSKFLSHWDHITRVKKPVIAAVNGYALG
  GGCELAMMCDIIYAGEKAQFGQPEILLGTIPRAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVS
  KIFPVEKLVEEAIQCAEKIASNSKIVVAMAKESVNAAFEMTLTEGNKLEKRLFYSTFATDDRREGMTAFV
  EKRKANFKDH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053119
• ORF Size: 873 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053119.1, NM_053119.2, NP_444349.1
• RefSeq Size: 1491 bp
• RefSeq ORF: 873 bp
• Locus ID: 93747
• MW: 31.6 kDa