Otud6b (BC031474) Mouse Tagged ORF Clone

CAT#: MR204044

Otud6b (Myc-DDK-tagged) - Mouse OTU domain containing 6B (cDNA clone MGC:27712 IMAGE:2599116)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Otud6b
• Synonyms: 2600013N14Rik|AU015433
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR204044 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGAGGTCGTGGCTGAAGAGCTCGATGATGAGGAGCAGCTGGTGAGAAGGCATCGGAAGGAGAAGA
  AGGAGCTGCAAGCCAAAATTCAGGGAATGAAGAACGCTGTTCCCAAAAACGACAAAAAGAGGAGGAAGCA
  GCTCACGGAAGATGTTGCTAAATTGGAAAGAGAAATGGAGCAAAAACACAGGGAAGAACTGGAGCAATTG
  AAGCAATTGACTTTCAAGGACAGTAAGATAGATTCTGTTGCTGTTAACATTTCAAACTTGGTACTTGAGA
  ATCAACCACCTCGGATTTCAAAAGCACAAAAGAGACGGGAAAAGAAGGCTGCATTGGAAAAGGAGCGGGA
  AGAAAGGATAGCAGAGGCTGAAATTGAGAACTTATCTGGAGCCAGACATCTTGAGAGTGAAAAACTTGCT
  CAAATATTGGCAGCCAGAGAATTGGAAATTAAACAGATTCCATCTGATGGTCACTGTATGTATGGAGCAC
  TTGAGGATCAGCTGAGAGAACAAGACTGTGCGCTGACTGTGGCTTCCCTGAGGAGGCAGACTGCTGAGTA
  CATGCAAACCCATTCAGACGACTTCCTGCCGTTTCTAACAAACCCCAGCACAGGAGATATGTACACGCCA
  GAAGAGTTTGGGAAGTACTGTGACGATATTGTGAACACAGCGGCATGGGGCGGTCAGCTCGAGCTAAGAG
  CTTTGTCTCACATCTTACAAACACCAATCGAGATACTACAGGCAGATGCTCCTCCTATTATAGTCGGTGA
  AGAGTATCCGAGGAATCCATTAGTACTAGTATACATGAGGCACGCGTATGGCTTAGGAGAACATTATAAT
  TCTGTTACACGGTTGGTGAACTCAGCTACTGAAAATTGCAGC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR204044 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEEVVAEELDDEEQLVRRHRKEKKELQAKIQGMKNAVPKNDKKRRKQLTEDVAKLEREMEQKHREELEQL
  KQLTFKDSKIDSVAVNISNLVLENQPPRISKAQKRREKKAALEKEREERIAEAEIENLSGARHLESEKLA
  QILAARELEIKQIPSDGHCMYGALEDQLREQDCALTVASLRRQTAEYMQTHSDDFLPFLTNPSTGDMYTP
  EEFGKYCDDIVNTAAWGGQLELRALSHILQTPIEILQADAPPIIVGEEYPRNPLVLVYMRHAYGLGEHYN
  SVTRLVNSATENCS
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: BC031474
• ORF Size: 882 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC031474.1
• RefSeq Size: 2598 bp
• RefSeq ORF: 884 bp
• Locus ID: 72201
• MW: 33.8 kDa