Pim2 (BC027376) Mouse Tagged ORF Clone

CAT#: MR204426

Pim2 (Myc-DDK-tagged) - Mouse proviral integration site 2 (cDNA clone MGC:37188 IMAGE:4954711)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pim2
• Synonyms: DXCch3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR204426 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGACCAAGCCTCTGCAGGGGCATCCTTCGCCCCCTGTGACCCCCACGCAGCCTCCAGGAGGCAAGG
  ATCGGGCAGCTTTCGAGGCCGAATACCGACTTGGCCCCCTCCTGGGTAAGGGAGGCTTTGGCACCGTCTT
  CGCGGGACACCGCGTCACGGATAGACGTCAGGTGGCCATCAAAGTAATCTCCCGGAACCGTGTGCTAGGC
  TGGTCCACCGTGTCAGACTCAGTCACCTGCCCACTTGAGGTTGCGCTGCTGTGGAAGGTGGGTGAAGGCA
  ATGGCCATCCGGGTGTGATACGCCTTCTTGACTGGTTCGAAACACCCGAAGGCTTCATGCTGGTCCTTGA
  GCGGCCTATGCCTGCTCAGGATCTCTTCGACTATATCACAGAGAAGGGGCCGCTGGGTGAAAGCTGTAGC
  CGCAGCTTCTTTACCCAAGTCGTGGCAGCTGTCCAGCACTGCCACGCCCGTGGAGTTGTCCATCGGGATA
  TCAAGGATGAGAACATCCTGATCGACCTATGCCGGGGTTCCATTAAACTCATTGATTTTGGTTCCGGCGC
  CCTGCTTCACGATGAGCCGTACACTGACTTTGATGGGACAAGAGTGTATAGCCCTCCAGAGTGGATCTCG
  CGACACCAGTACCATGCCCTGCCAGCGACCGTCTGGTCACTAGGTGTCCTACTCTATGACATGGTCTGTG
  GGGACATTCCCTTCGAGAGAGACCAGGAGATTCTGGAGGCTGAGCTGCACTTCCCTGCTCATGTCTCCCC
  AGATTGCTGTGCCCTAATCCGCCGGTGCCTGGCCCCTAAACCCTGCTCCCGACCCTCACTGGAGGAGATT
  CTGCTGGACCCCTGGATGCAATCACCAGCTGAAGAAAAGCCCATCAACTCCTCCAAAGGAAGCCCCACCC
  CCTTGCCCTGGTCCCTGCTTCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR204426 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLTKPLQGHPSPPVTPTQPPGGKDRAAFEAEYRLGPLLGKGGFGTVFAGHRVTDRRQVAIKVISRNRVLG
  WSTVSDSVTCPLEVALLWKVGEGNGHPGVIRLLDWFETPEGFMLVLERPMPAQDLFDYITEKGPLGESCS
  RSFFTQVVAAVQHCHARGVVHRDIKDENILIDLCRGSIKLIDFGSGALLHDEPYTDFDGTRVYSPPEWIS
  RHQYHALPATVWSLGVLLYDMVCGDIPFERDQEILEAELHFPAHVSPDCCALIRRCLAPKPCSRPSLEEI
  LLDPWMQSPAEEKPINSSKGSPTPLPWSLLP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC027376
• ORF Size: 933 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC027376, AAH27376
• RefSeq Size: 1974 bp
• RefSeq ORF: 935 bp
• Locus ID: 18715
• MW: 34.5 kDa