Calhm6 (NM_175449) Mouse Tagged ORF Clone

CAT#: MR204443

Fam26f (Myc-DDK-tagged) - Mouse family with sequence similarity 26, member F (Fam26f)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Calhm6
• Synonyms: A630077B13Rik; Fam26f
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR204443 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAAAGTTCAAGGCAGTGCTGGACCTGCAGAGAAAGCACCGCAACGCCCTGGGCTATAGCCTGGTGA
  CCCTACTGACGGCTGGTGGGGAGAAGATCTTCTCCTCAGTGGTGTTCCAGTGTCCCTGCACTGCCACCTG
  GAACCTGCCCTACGGCCTGGTGTTCCTGCTGGTGCCTGCCCTCGCGCTTTTCCTCCTGGGATATGCGCTG
  AGCGCGCGCACATGGCGCCTGCTCACCGGCTGCTGCTCCCGGAGCGCGCGATTCAGTTCGGGGTTGCGCA
  GCGCGTTCGTGTGCGCCCAGCTCAGCATGACCGCGGCATTCGCGCCCCTCACCTGGGTGGCCGTGGCGCT
  GCTCGAGGGCTCTTTCTACCAATGTGCTGTCAGCGGGAGCGCGCGCTTGGCGCCATACCTGTGCAAGGGC
  CGCGACCCCAACTGCAATGCCACGCTACCGCAGGCTCCCTGCAACAAGCAGAAGGTGGAAATGCAGGAGA
  TCCTGAGCCAGCTCAAGGCTCAGTCTCAGGTGTTCGGTTGGATTCTGATAGCTGCCGTTATTATCTTACT
  TCTTCTTGTTAAGTCTGTGACCCGATGCTTCTCTCCGGTTAGTTATCTGCAGTTAAAATTCTGGGAAATC
  TATTGGGAAAAGGAGAAGCAGATTCTTCAAAATCAAGCTGCAGAGAATGCGACACAGTTGGCCGAAGAGA
  ATGTTAGATGTTTCTTTGAGTGCTCGAAGCCGAAGGAATGCAACACTCCAAGCAGTAAAGACTGGCAGGA
  AATCTCAGCGTTGTACACATTCAATCCCAAGAACCAGTTCTACAGCATGCTGCACAAGTATGTTAGCAGA
  GAAGAAATGAGCGGCAGTGTCCGCTCTGTGGAAGGAGATGCAGTGATCCCTGCCCTTGGCTTTGTAGATG
  ACATGTCCATGACTAACACTCACGAACTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR204443 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEKFKAVLDLQRKHRNALGYSLVTLLTAGGEKIFSSVVFQCPCTATWNLPYGLVFLLVPALALFLLGYAL
  SARTWRLLTGCCSRSARFSSGLRSAFVCAQLSMTAAFAPLTWVAVALLEGSFYQCAVSGSARLAPYLCKG
  RDPNCNATLPQAPCNKQKVEMQEILSQLKAQSQVFGWILIAAVIILLLLVKSVTRCFSPVSYLQLKFWEI
  YWEKEKQILQNQAAENATQLAEENVRCFFECSKPKECNTPSSKDWQEISALYTFNPKNQFYSMLHKYVSR
  EEMSGSVRSVEGDAVIPALGFVDDMSMTNTHEL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_175449
• ORF Size: 942 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175449.2, NM_175449.3, NM_175449.4, NP_780658.2
• RefSeq Size: 1095 bp
• RefSeq ORF: 942 bp
• Locus ID: 215900
• MW: 35 kDa