Qpct (BC020023) Mouse Tagged ORF Clone

CAT#: MR204454

Qpct (Myc-DDK-tagged) - Mouse glutaminyl-peptide cyclotransferase (glutaminyl cyclase) (cDNA clone MGC:27858 IMAGE:3491756)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Qpct
• Synonyms: 5730422A13Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR204454 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGGCAGCGAAGACAAACGCGTCGTGGGCACTCTCCACCTGCTGCTACTGCAGGCGACCGTCCTGT
  CTCTGACAGCTGGGAATCTGAATCTGGTCTCCGGTGCCTGGACGCAGGAGAAGCACATCATGCAACGAAT
  TCAGAGACTTCAGGCTGAGTGGGTCGTGGAAGTTGACACCTTCCTGAGTAGGACTCCCTATGGCTATCGG
  TCCTTCTCAAATATCATCAGCACTCTTAACCCGGAAGCGAAACGACACCTGGTCCTCGCCTGCCACTACG
  ACTCCAAATATTTTCCTCGATGGGACAGCAGAGTGTTTGTGGGAGCCACGGATTCAGCTGTGCCATGTGC
  AATGATGTTGGAACTTGCCCGTGCCTTAGACAAGAAACTCCATTCCTTGAAGGATGTCTCTGGTTCCAAG
  CCAGATCTCTCACTCCGGCTAATTTTCTTTGATGGTGAAGAGGCTTTTCATCACTGGTCCCCTCAAGATT
  CTCTGTATGGGTCTCGGCACTTAGCTCAGAAGATGGCATCAAGCCCTCACCCTCCTGGATCAAGAGGCAC
  CAACCAACTGGATGGCATGGATCTGTTGGTCTTACTAGATTTAATTGGAGCAGCAAATCCAACATTCCCT
  AATTTTTTCCCCAAGACTACCAGATGGTTTAATAGACTTCAAGCAATTGAAAAGGAACTCTATGAATTGG
  GATTACTCAAGGATCATTCTTTGGAGAGGAAGTATTTTCAGAATTTTGGCTATGGAAATATTATCCAGGA
  TGACCACATTCCATTTTTAAGAAAAGGTGTCCCAGTTCTTCACCTGATAGCTTCTCCTTTCCCTGAAGTC
  TGGCACACCATGGATGACAATGAAGAAAATCTACATGCGTCAACCATTGACAATCTCAACAAAATCATTC
  AAGTCTTTGTGTTGGAATATCTTCACTTG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR204454 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAGSEDKRVVGTLHLLLLQATVLSLTAGNLNLVSGAWTQEKHIMQRIQRLQAEWVVEVDTFLSRTPYGYR
  SFSNIISTLNPEAKRHLVLACHYDSKYFPRWDSRVFVGATDSAVPCAMMLELARALDKKLHSLKDVSGSK
  PDLSLRLIFFDGEEAFHHWSPQDSLYGSRHLAQKMASSPHPPGSRGTNQLDGMDLLVLLDLIGAANPTFP
  NFFPKTTRWFNRLQAIEKELYELGLLKDHSLERKYFQNFGYGNIIQDDHIPFLRKGVPVLHLIASPFPEV
  WHTMDDNEENLHASTIDNLNKIIQVFVLEYLHL
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: BC020023
• ORF Size: 939 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC020023, AAH20023
• RefSeq Size: 1486 bp
• RefSeq ORF: 941 bp
• Locus ID: 70536
• MW: 35.7 kDa