Nhsl1 (BC013565) Mouse Tagged ORF Clone

CAT#: MR204513

Nhsl1 (Myc-DDK-tagged) - Mouse NHS-like 1 (cDNA clone MGC:8247 IMAGE:3591967)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nhsl1
• Synonyms: A730096F01, mKIAA1357
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR204513 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCCAACCTAATACATCACCGGGCCCCACACAGGAAGAGTCAGGCGAGAACAGTGTGGATGGTGAGA
  GAAATGCGAAGAGCTGCCTGTCTCAACAGGGCAGAGAAGCTGGCTTGCTGGAACCCAACACAGCTGCTTC
  CTCTTCAGACCCCGTGGATGTATCTAAGGAAGAGGGAAGCGATGAGGTGCTGACTCCGACTAAACCCAGG
  ACAACAGAAGACCTGTTTGCAGCCATTCACAGATCTAAAAGGAAAGTCCTTGGCCGGAAAGACTCAGAAG
  ACGATCACACTCGAAATCACTCTCCCTCCCCGCCTGTGACACCCACGAGTGCTGCCCCTAACCTGGCCTC
  TCCAAAGCAAGTAGGGTCTATCCAGAGAAGCATAAAAAAGAGCACTACCAGCAGTGACAACTTCAAAGCT
  CTGCTGCTAAAGAAGGGGAGTCGCTCGGACACCAGTGCTCGCATGTCAGCGGCTGAGATGCTCAAGTCTA
  CGGACCCTCGCTTCCAGAGATCTAGGTCGGAGCCTTCGGCAGACAGCCCGGACAGCCCATCCAGCTGCTC
  CCCCAACAAGAACAGGAGAGCTCAGGAAGAGTGGGCCAAGAACGAAGGCCTGATGCCTCGGAGTCTGTCC
  TTTTCGGGCCCTCGGTACAGCCGCAGCAGGACGCCACCCTCTGCGGCCAGCAGCAGGTACAGCATGCGGA
  ACCGCATCCAGAGCAGCCCCATGACCGTCATCTCCGAGGGAGAGGGAGAACCTGCTGAGCCAGCGGACAA
  CAAAGCACGCAGGGCCCTGGATGCTACCAGGGTGTGCTCTCTGGACAGACTGACGGGTCAGGAGATGGAC
  CAGGCCAGCCTCCTCTGTAGCGAGGAGCCTGCCTCTGTGGATGGGATAGGAAGGGCTGAGGGCAACGGTC
  CCTCGGAGCAGTGTGGGGGTATGGAACAGAAGAGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR204513 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVQPNTSPGPTQEESGENSVDGERNAKSCLSQQGREAGLLEPNTAASSSDPVDVSKEEGSDEVLTPTKPR
  TTEDLFAAIHRSKRKVLGRKDSEDDHTRNHSPSPPVTPTSAAPNLASPKQVGSIQRSIKKSTTSSDNFKA
  LLLKKGSRSDTSARMSAAEMLKSTDPRFQRSRSEPSADSPDSPSSCSPNKNRRAQEEWAKNEGLMPRSLS
  FSGPRYSRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEPAEPADNKARRALDATRVCSLDRLTGQEMD
  QASLLCSEEPASVDGIGRAEGNGPSEQCGGMEQKS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC013565
• ORF Size: 945 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC013565, AAH13565
• RefSeq Size: 2934 bp
• RefSeq ORF: 947 bp
• Locus ID: 215819
• MW: 33.8 kDa