Slc9a3r2 (BC070458) Mouse Tagged ORF Clone

CAT#: MR204526

Slc9a3r2 (Myc-DDK-tagged) - Mouse solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulator 2 (cDNA clone MGC:96425 IMAGE:30603578)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Slc9a3r2
• Synonyms: E3karp, Sip-1, Tka-1, Octs2, NHERF-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR204526 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGCGCTGGAATCGCTGCGGCCCCGCTTGTGTCGCCTGGTGCGCGGAGAGCAAGGGTACGGCTTTC
  ACCTGCATGGCGAGAAAGGCCGCCGTGGCCAGTTCATCCGGCGCGTGGAGCCAGGCTCCCCAGCCGAGGC
  GGCCGCGCTGCGCGCCGGGGACCGCCTGGTTGAGGTCAACGGCGTCAACGTAGAGGGCGAGACGCATCAC
  CAGGTGGTACAGAGGATCAAGGCTGTGGAGGGGCAGACCCAGCTGCTTGTGGTGGACAAGGAGACCGATG
  AGGAGCTCTGCCGGCGGCAACTGACCTGCACTGAGGAGATGGCCCATCGAGGGCTTCCCCCAGCCCACAA
  CCCCTGGGAGCCAAAGCCGGACTGGGCATGCTCGGGCAGCCTTGGCTCGGACACTGGCCAGAAGGATGTC
  AATGGGCCCCCAAGAGAGCTGCGTCCCCGGCTCTGCCACTTGCGAAGGGGACCCCAGGGCTACGGGTTCA
  ACCTGCATAGTGACAAGTCCCGGCCTGGCCAGTACATCCGCTCTGTGGACCCTGGCTCTCCTGCTTCTCA
  CTCTGGCCTCCGTGCCCAGGATCGGCTCATTGAGGTGAATGGGCAGAACGTGGAGGGGCTGCGACATGCC
  GAGGTTGTCGCCAGAATCAAGGCACAGGAGGACGAGGCCCGGCTGCTGGTAGTCGATCCTGAGACTGATG
  AACACTTCAAGCGGTTGAGGGTCATACCCACTGAGGAACATGTAGAAGGTCCACTGCCATCACCAGTCAC
  GAACGGGACCAGTCCTGCCCAGGATGGCAGTACCTGGAAGCGAGACCCCTTCCAGGAAAGTGGCCTCCAC
  CTGAGTCCCACAGCAGCTGAGGCCAAGGAGAAGGCTCGAGCTACTCGGGTCAACAAGCGGGCACCACAGA
  TGGACTGGAACCGGAAGCGAGAGATCTTCAGCAACTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR204526 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAALESLRPRLCRLVRGEQGYGFHLHGEKGRRGQFIRRVEPGSPAEAAALRAGDRLVEVNGVNVEGETHH
  QVVQRIKAVEGQTQLLVVDKETDEELCRRQLTCTEEMAHRGLPPAHNPWEPKPDWACSGSLGSDTGQKDV
  NGPPRELRPRLCHLRRGPQGYGFNLHSDKSRPGQYIRSVDPGSPASHSGLRAQDRLIEVNGQNVEGLRHA
  EVVARIKAQEDEARLLVVDPETDEHFKRLRVIPTEEHVEGPLPSPVTNGTSPAQDGSTWKRDPFQESGLH
  LSPTAAEAKEKARATRVNKRAPQMDWNRKREIFSNF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC070458
• ORF Size: 948 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC070458, AAH70458
• RefSeq Size: 2052 bp
• RefSeq ORF: 950 bp
• Locus ID: 65962
• MW: 35.3 kDa