Eef1g (BC023495) Mouse Tagged ORF Clone

CAT#: MR204550

Eef1g (Myc-DDK-tagged) - Mouse eukaryotic translation elongation factor 1 gamma (cDNA clone MGC:30982 IMAGE:5251765)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Eef1g
• Synonyms: EF1G
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR204550 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACCACAACAAACAGGCCACTGAAAATGCGAAAGAGGAGGTGAAGCGAATCCTGGGGCTGCTGGACA
  CTCACTTGAAGACTCGAACTTTTCTAGTGGGCGAGCGAGTGACACTGGCTGATATTACAGTTGTCTGTAC
  CCTGTTGTGGCTCTATAAACAGGTCTTAGAGCCTTCTTTTCGCCAGGCCTTCCCCAATACCAACCGGTGG
  TTCCTTACCTGCATTAACCAGCCCCAGTTCAGGGCTATCTTGGGGGAGGTGAAGCTGTGTGAGAAGATGG
  CTCAGTTTGATGCTAAGAAGTTTGCAGAGAGCCAGCCTAAAAAAGATACCCCAAGGAAAGAAAAGGGTTC
  ACGAGAGGAGAAACAGAAACCCCAGGCTGAGCGGAAGGAGGAAAAAAAGGCAGCTGCCCCAGCTCCTGAG
  GAAGAGATGGATGAGTGTGAGCAGGCATTGGCTGCTGAGCCCAAGGCCAAGGACCCTTTCGCTCACCTGC
  CCAAGAGTACCTTTGTGTTGGATGAGTTTAAGCGTAAGTACTCCAATGAGGACACCCTCTCTGTGGCACT
  GCCATATTTTTGGGAGCACTTTGATAAAGATGGCTGGTCCCTGTGGTATGCCGAGTACCGCTTCCCTGAA
  GAGCTGACCCAGACCTTCATGAGTTGCAACCTCATCACTGGGATGTTTCAGCGATTGGACAAACTGAGGA
  AGAATGCCTTTGCTAGTGTTATCCTCTTTGGAACCAACAACAGCAGCTCCATTTCTGGTGTTTGGGTCTT
  CCGAGGCCAAGAGCTTGCCTTTCCGCTGAGTCCAGATTGGCAGGTGGACTATGAGTCGTATACATGGCGG
  AAACTGGATCCTGGGAGCGAGGAAACCCAGACCCTGGTTCGAGAGTACTTTTCCTGGGAGGGGACCTTCC
  AGCATGTGGGCAAAGCCGTCAATCAAGGCAAGATCTTCAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR204550 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MHHNKQATENAKEEVKRILGLLDTHLKTRTFLVGERVTLADITVVCTLLWLYKQVLEPSFRQAFPNTNRW
  FLTCINQPQFRAILGEVKLCEKMAQFDAKKFAESQPKKDTPRKEKGSREEKQKPQAERKEEKKAAAPAPE
  EEMDECEQALAAEPKAKDPFAHLPKSTFVLDEFKRKYSNEDTLSVALPYFWEHFDKDGWSLWYAEYRFPE
  ELTQTFMSCNLITGMFQRLDKLRKNAFASVILFGTNNSSSISGVWVFRGQELAFPLSPDWQVDYESYTWR
  KLDPGSEETQTLVREYFSWEGTFQHVGKAVNQGKIFK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC023495
• ORF Size: 951 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC023495, AAH23495
• RefSeq Size: 1391 bp
• RefSeq ORF: 953 bp
• Locus ID: 67160
• MW: 36.9 kDa