Trib3 (BC012955) Mouse Tagged ORF Clone

CAT#: MR204806

  • TrueORF®

Trib3 (Myc-DDK-tagged) - Mouse tribbles homolog 3 (Drosophila) (cDNA clone MGC:18731 IMAGE:3980838)


  "BC012955" in other vectors (4)


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Reconstitution Protocol

USD 68.00

USD 670.00

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Size
    • 10 ug

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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Trib3
Synonyms Trb3, Nipk, SINK, SKIP3, TRB-3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR204806 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGAGCTACACCTCTGGCTGCTTCTGCTGATGTTTCCTGCAGGAAGAAACCGTTGGAGTTTGATGACA
ATATTGATGCCAAGTGTCCAGTCCTAAAACGAGTGAGAGATGAGCCTGAGCCCGGACCACTCCCCAGCCT
GCTGCCCCCCAGCCCACCTCCCGCCTCAGACTTGTCACCTGCTGTGGCCCCTGCAACTCGACTGGGGCCT
TATATCCTTTTGGAACGAGAGCAAGGCAGCTGCAGCTATCGAGCCCTGCACTGCCCCACAGGCACAGAGT
ACACCTGCAAGGTGTACCCTGCCAGCGAGGCCCAGGCGGTGCTGGCACCTTATGCCCGGCTGCCTACCCA
CCAGCATGTGGCCCGTCCCACAGAGGTCCTGCTGGGCTCTCGGCTCCTTTACATCTTTTTCACGAAGACC
CATGGGGACTTGCACAGCCTGGTGCGCAGCCGCCGCGGTATCCCAGAGCCCGAGGCTGCCGGGCTCTTCC
GGCAGATGGCTAGTGCCGTGGCACACTGCCACAAGCACGGGCTTGTCTTGCGCGACCTCAAGCTGCGTCG
CTTTGTCTTCAGCAACTGTGAGAGGACGAAGCTGGTGCTGGAGAACCTGGAAGATGCCTGCGTGATGACT
GGATCAGATGACTCTCTGTGGGACAAGCATGCGTGCCCTGCCTACGTGGGACCAGAGATACTCAGCTCCC
GGCCATCCTACTCTGAGCCAGTCCTGCTCTTTGGCAAGATCCGTAGAGGGACCTTTGCCCTGCCTGAGGG
CCTATCAGCCCCAGCCCGCTGTCTGATCCGCTGTCTCCTCCGCAAGGAACCTTCAGAGCGACTTGTGGCC
CTGGGAATCCTCTTGCATCCCTGGTTGAGAGAGGATCACGGCCGAGTCTCTCCTCCACAGTCTGACCGAA
GGGAGATGGACCAGGTGGTCCCAGATGGGCCACAGCTGGAGGAGGCTGAGGAAGGGGAGGTGGGGCTGTA
CGGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR204806 protein sequence
Red=Cloning site Green=Tags(s)

MRATPLAASADVSCRKKPLEFDDNIDAKCPVLKRVRDEPEPGPLPSLLPPSPPPASDLSPAVAPATRLGP
YILLEREQGSCSYRALHCPTGTEYTCKVYPASEAQAVLAPYARLPTHQHVARPTEVLLGSRLLYIFFTKT
HGDLHSLVRSRRGIPEPEAAGLFRQMASAVAHCHKHGLVLRDLKLRRFVFSNCERTKLVLENLEDACVMT
GSDDSLWDKHACPAYVGPEILSSRPSYSEPVLLFGKIRRGTFALPEGLSAPARCLIRCLLRKEPSERLVA
LGILLHPWLREDHGRVSPPQSDRREMDQVVPDGPQLEEAEEGEVGLYG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC012955
ORF Size 984 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq BC012955, AAH12955
RefSeq Size 1969 bp
RefSeq ORF 986 bp
Locus ID 228775
MW 36.2 kDa

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