Rad51d (NM_011235) Mouse Tagged ORF Clone

CAT#: MR204841

Rad51d (Myc-DDK-tagged) - Mouse RAD51-like 3 (S. cerevisiae) (Rad51l3)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rad51d
• Synonyms: R51H3; Rad51l3; TRAD; Trad-d5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR204841 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCATGCTCAGGGCAGGGCTGTGCCCGGGCCTCACCGAGGAGACCGTCCAGCTTCTCAGAGGCCGAA
  AGATAAAAACAGTGGCAGACCTGGCAGCTGCTGACTTGGAGGAAGTAGCCCAGAAGTGTGGCTTGTCCTA
  CAAGGCCCTCGTTGCCCTGAGGAGGGTGTTGCTGGCGCAGTTCTCGGCTTTCCCCTTAAATGGCGCAGAT
  CTCTATGAGGAACTGAAGACTTCCACGGCCATCCTGTCCACCGGCATCGGAAGCCTGGACAAACTACTTG
  ATGCTGGCCTCTATACTGGGGAGGTGACTGAAATTGTGGGTGGCCCAGGTAGCGGCAAAACCCAGGTGTG
  TCTCTGTGTGGCTGCAAATGTGGCCCATAGCCTGCAGCAGAATGTACTGTATGTGGATTCCAATGGAGGA
  ATGACGGCGTCCCGCCTCCTCCAGCTACTACAGGCTAGAACCCAAGATGAGGAGAAACAGGCAAGTGCTC
  TCCAGAGGATACAGGTGGTGCGTTCATTTGACATCTTCCGGATGCTAGATATGCTACAGGACCTTCGCGG
  CACCATAGCCCAGCAGGAAGCAACTTCTTCAGGCGCCGTGAAGGTTGTGATTGTGGACTCGGTCACTGCA
  GTGGTCGCCCCACTTCTGGGAGGTCAGCAGAGGGAAGGCCTGGCCTTGATGATGCAGCTGGCCCGAGAGC
  TCAAGATCCTGGCCCGGGACCTGGGTGTGGCAGTGGTGGTGACCAACCACTTGACTCGAGATTGGGATGG
  TAGAAGATTCAAACCTGCCCTTGGACGCTCCTGGAGCTTTGTGCCCAGTACCCGGATTCTCCTGGATGTC
  ACTGAGGGGGCTGGGACACTCGGTAGCAGCCAACGCACAGTATGTCTGACCAAGTCTCCCCGCCAGCCAA
  CGGGTCTGCAGGAGATGATAGACATTGGGACATTGGGGACTGAGGAGCAGAGCCCAGAATTACCTGGCAA
  GCAGACG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR204841 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGMLRAGLCPGLTEETVQLLRGRKIKTVADLAAADLEEVAQKCGLSYKALVALRRVLLAQFSAFPLNGAD
  LYEELKTSTAILSTGIGSLDKLLDAGLYTGEVTEIVGGPGSGKTQVCLCVAANVAHSLQQNVLYVDSNGG
  MTASRLLQLLQARTQDEEKQASALQRIQVVRSFDIFRMLDMLQDLRGTIAQQEATSSGAVKVVIVDSVTA
  VVAPLLGGQQREGLALMMQLARELKILARDLGVAVVVTNHLTRDWDGRRFKPALGRSWSFVPSTRILLDV
  TEGAGTLGSSQRTVCLTKSPRQPTGLQEMIDIGTLGTEEQSPELPGKQT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011235
• ORF Size: 990 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011235.1, NM_011235.2, NM_011235.3, NM_011235.4, NP_035365.1
• RefSeq Size: 7072 bp
• RefSeq ORF: 990 bp
• Locus ID: 19364
• MW: 35.3 kDa
• Gene Summary: This gene belongs to the Rad51 gene family whose products play a major role in homologous recombination and DNA repair. The encoded protein interacts with other proteins of this family, including Rad51b, Rad51c and Xrcc2, and plays an essential role in both DNA repair and telomere maintenance. In humans, germline mutations in this gene may be associated with predisposition to ovarian cancer. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2013]