Extl2 (NM_021388) Mouse Tagged ORF Clone

CAT#: MR204853

Extl2 (Myc-DDK-tagged) - Mouse exostoses (multiple)-like 2 (Extl2), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Extl2
• Synonyms: 3000001D04Rik; AW146439
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR204853 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGAGGGGTTGCCACATCTGCAAACTTCCGGGAAGAGTCATGGGGATTCGAGTGCTCCGGTTCTCCC
  TGGTGGTCATCCTTGTGTTGCTGCTGGTAGCGGGGGCTTTGACCAACTTACTCCCCAACATCAAAGAAGA
  CAAGATGCTCACTCTGCGTAGGGAGATCAAATCCCCGAGCAAGTCGGCCCTGGATTCATTCACCCTCATC
  ATGCAGACATACAACAGAACAGACCTCTTACTAAGACTCTTAAATCATTACCAGGCAGTACCGAGTCTGC
  ACAAGGTGATCGTGGTGTGGAACAATGTGGGAGAGAAGGGCCCAGAGGAGTTGTGGAATTCTCTAGGGCC
  TCACCCGATCCCTGTCATCTTCAAACCACAAACAGCAAACAAAATGAGAAACCGACTCCAGGTCTTCCCT
  GAAGTGGAGACCAACGCGGTGTTGATGGTAGACGATGACACACTCATTAGTGCCCAGGACCTTGTTTTCG
  CTTTCTCAATTTGGCAGCAATTTCCTGATCAGATCATAGGATTTGTACCTAGAAAGCACGTCTCTACTTC
  CTCCGGGATCTACAGTTACGGAGGCTTTGAGCTGCAGACACCGGGGCCCGGGAATGGCGACCAGTACTCC
  ATGGTGCTCATCGGAGCCTCCTTCTTCAACAGCAAATACCTGGAACTCTTCCAGAAGCAGCCTGCAGCCG
  TCCACGCGCTGATCGATGAGACGCAGAACTGTGACGACATCGCTATGAATTTCCTCGTCACCAGGCACAC
  TGGCAAGCCTTCAGGGATATTCGTGAAACCCATAAACATGGTCAACTTGGAAAAAGAAACCAACGGGTAT
  TCCGGAATGTGGCACCGGGCGGAGCACTTTCTGCAGAGATCCTACTGTATAAATAAGCTCGTTAACATCT
  ACGACGGCATGCCCTTAAAGTACTCCAACATTATGATTTCCCAGTTTGGTTTTCCGTATGCCAACCATAA
  AAGTAAAATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR204853 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MMRGCHICKLPGRVMGIRVLRFSLVVILVLLLVAGALTNLLPNIKEDKMLTLRREIKSPSKSALDSFTLI
  MQTYNRTDLLLRLLNHYQAVPSLHKVIVVWNNVGEKGPEELWNSLGPHPIPVIFKPQTANKMRNRLQVFP
  EVETNAVLMVDDDTLISAQDLVFAFSIWQQFPDQIIGFVPRKHVSTSSGIYSYGGFELQTPGPGNGDQYS
  MVLIGASFFNSKYLELFQKQPAAVHALIDETQNCDDIAMNFLVTRHTGKPSGIFVKPINMVNLEKETNGY
  SGMWHRAEHFLQRSYCINKLVNIYDGMPLKYSNIMISQFGFPYANHKSKM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021388
• ORF Size: 993 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021388.1, NM_021388.2, NM_021388.3, NM_021388.4, NP_067363.2, NP_067363.3
• RefSeq Size: 3012 bp
• RefSeq ORF: 993 bp
• Locus ID: 58193
• MW: 37.4 kDa