B3galnt1 (NM_020026) Mouse Tagged ORF Clone

CAT#: MR204877

B3galnt1 (Myc-DDK-tagged) - Mouse UDP-GalNAc:betaGlcNAc beta 1,3-galactosaminyltransferase, polypeptide 1 (B3galnt1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: B3galnt1
• Synonyms: B3galt3; b3GT3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR204877 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCCGGCTGTCTTGACTGCCCTTCCCAATAGGATGTCACTGAGATCCCTCAAGTGGAGCCTGCTGC
  TGCTGTCCCTGCTGAGCTTCCTGGTGATATGGTACCTCAGCCTCCCCCACTACAATGTGATCGAGCGCGT
  CAACTGGATGTACTTCTATGAGTATGAGCCAATTTACAGACAAGACTTTCGCTTCACGCTCCGCGAACAC
  TCGAACTGTTCTCATCAGAACCCATTCCTGGTCATCCTGGTGACCTCACGCCCCTCAGATGTGAAAGCCA
  GACAAGCCATTAGAGTTACTTGGGGTGAGAAAAAGTCCTGGTGGGGATATGAGGTGCTCACGTTTTTCTT
  ACTAGGCCAGCAGGCTGAAAGGGAAGACAAAACGTTAGCCCTGTCCTTGGAGGATGAGCACGTTCTCTAT
  GGTGATATTATACGGCAAGACTTTCTAGACACATATAATAACTTGACCTTGAAAACCATTATGGCTTTCA
  GGTGGGTAATGGAGTTTTGCCCCAATGCCAAGTATATTATGAAAACAGACACTGATGTTTTCATCAACAC
  TGGCAATTTAGTCAAGTATCTTTTAAACCTAAACCACTCAGAGAAGTTTTTCACGGGCTATCCTCTAATT
  GATAACTATTCCTATAGAGGATTTTTCCATAAAAACCACATTTCATACCAAGAGTACCCCTTCAAGGTGT
  TCCCTCCCTACTGCAGCGGGCTGGGCTACATTATGTCCGGCGACCTGGTGCCCAGGGTCTACGAGATGAT
  GAGTCACGTGAAGCCCATCAAGTTTGAAGACGTTTATGTTGGCATCTGTTTGAATTTGTTAAAAGTGGAC
  ATTCATATTCCAGAAGACACAAACCTTTTCTTTCTGTACAGAATCCACTTGGATGTATGTCAGCTCAGAC
  GCGTGATTGCAGCCCATGGCTTTTCTTCCAAGGAGATCATCACATTCTGGCAGGTGATGCTGAGGAACAC
  CACATGCCATTAC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR204877 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAPAVLTALPNRMSLRSLKWSLLLLSLLSFLVIWYLSLPHYNVIERVNWMYFYEYEPIYRQDFRFTLREH
  SNCSHQNPFLVILVTSRPSDVKARQAIRVTWGEKKSWWGYEVLTFFLLGQQAEREDKTLALSLEDEHVLY
  GDIIRQDFLDTYNNLTLKTIMAFRWVMEFCPNAKYIMKTDTDVFINTGNLVKYLLNLNHSEKFFTGYPLI
  DNYSYRGFFHKNHISYQEYPFKVFPPYCSGLGYIMSGDLVPRVYEMMSHVKPIKFEDVYVGICLNLLKVD
  IHIPEDTNLFFLYRIHLDVCQLRRVIAAHGFSSKEIITFWQVMLRNTTCHY
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020026
• ORF Size: 996 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020026.1, NM_020026.2, NM_020026.3, NM_020026.4, NP_064410.1
• RefSeq Size: 2283 bp
• RefSeq ORF: 996 bp
• Locus ID: 26879
• MW: 39.4 kDa