H2 (BC003819) Mouse Tagged ORF Clone

CAT#: MR205178

H2 (Myc-DDK-tagged) - Mouse histocompatibility 2, T region locus 22 (cDNA clone MGC:6180 IMAGE:3484613)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: H2
• Synonyms: 27b|H-2T17|H-2T22|H2-T17|T22b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR205178 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCTGGGTCCTCAGGGCCGCTGTGGTCTGCGCCCTTCTCCTGCAGCTGGATGCCAGACCATCCTGGA
  CTCGGATCCCTTTGGGTTCACACTCGCTTAGGTATTTCTACACCGCTGTGTCCCGGCCTGGCCTTGGGGA
  GCCCTGGTTCATAATCGTCGGCTATGTGGACGACATGCAGGTCCTGCGCTTCAGCAGCAAGGAGGAGACT
  CCGAGGATGGCACCCTGGCTGGAGCAGGAGGAAGCAGATAACTGGGAGCAGCAGACTCGTATAGTCACAA
  TTCAAGGACAGCTGTCTGAAAGGAATCTGATGACCCTGGTTCATTTTTACAACAAGAGCATGGACGACTC
  TCACACACTACAGTGGCTGCAAGGCTGCGATGTGGAGCCAGATCGGCACCTGTGTCTCTGGTACAACCAG
  CTCGCCTATGATAGCGAGGATCTCCCCACCCTGAACGAAAACCCAAGTTCCTGTACAGTGGGAAACAGCA
  CTGTACCTCACATCTCTCAGGACCTGAAGAGCCACTGCTCAGATCTGCTGCAGAAATACCTGGAAAAAGG
  GAAGGAGAGGCTGCTGCGTTCAGACCCTCCAAAGGCACATGTGACCCGTCACCCCAGACCTGAAGGTGAT
  GTCACCCTCAGGTGTTGGGCCCTGGGCTTCTACCCTGCTGACATCACCCTGACCTGGCAGTTGAATGGGG
  AGGAGCTGACCCAGGACATGGAGCTTGTGGAGACCAGGCCTGCAGGGGATGGAACCTTCCAGAAGTGGGC
  AGCTGTGGTGGTGCCTCTTGGGAAAGAGCAGAGTTACACATGCCATGTGTACCATGAGGGGCTGCCTGAG
  CCCCTCATCCTGAGATGGGGTGGGAGAGGAAGGCGTGACACCCAAGAAGCAGGCAGAGACAGTCCCCAAG
  ACTCTAGCAAGACTGTTGTGGATGATGAGGAGATGGGGGTTTGCTTTTGGAAGATTAAGTCCTGTAAAAC
  TTGTCTAGGCCACTCCCCAGGAACTTCAGTTGGCGAGTCTTTACTGTCACCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR205178 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSWVLRAAVVCALLLQLDARPSWTRIPLGSHSLRYFYTAVSRPGLGEPWFIIVGYVDDMQVLRFSSKEET
  PRMAPWLEQEEADNWEQQTRIVTIQGQLSERNLMTLVHFYNKSMDDSHTLQWLQGCDVEPDRHLCLWYNQ
  LAYDSEDLPTLNENPSSCTVGNSTVPHISQDLKSHCSDLLQKYLEKGKERLLRSDPPKAHVTRHPRPEGD
  VTLRCWALGFYPADITLTWQLNGEELTQDMELVETRPAGDGTFQKWAAVVVPLGKEQSYTCHVYHEGLPE
  PLILRWGGRGRRDTQEAGRDSPQDSSKTVVDDEEMGVCFWKIKSCKTCLGHSPGTSVGESLLSP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC003819
• ORF Size: 1032 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC003819, AAH03819
• RefSeq Size: 1547 bp
• RefSeq ORF: 1034 bp
• Locus ID: 15039
• MW: 39.1 kDa