Cd72 (BC003824) Mouse Tagged ORF Clone

CAT#: MR205247

Cd72 (Myc-DDK-tagged) - Mouse CD72 antigen (cDNA clone MGC:6220 IMAGE:3599554)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cd72
• Synonyms: CD72c|Ly-19|Ly-32|Ly-m19|Lyb-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR205247 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGACGCTATCACGTATGCAGACCTGCGCTTTGTGAAAGTGCCCCTGAAGAACAGTGCATCTAACC
  ATCTAGGACAGGACTGTGAGGCCTATGAAGATGGGGAACTCACCTACGAGAACGTGCAAGTGTCTCCAGT
  CCCAGGAGGGCCACCAGGCTTGGCTTCCCCTGCACTAGCGGACAAAGCAGGGGTCGGGTCAGAGCAACCA
  ACTGCGACCTGGAGCTCTGTGAAGTCGTCTGCTCTCAGGCAGATTCCCCGCTGTCCTACAGTCTGCTTGC
  AATACTTCTTGCTTGGCCTTCTCGTGTCCTGTCTGATGTTAGGGGTGGCTGTCATCTGCCTGGGAGTTCG
  CTATCTGCAGGTGTCTCGGCAGTTCCAGGAGGGGACCAGGATTTGGGAAGCCACCAATAGCAGCCTGCAG
  CAGCAGCTCAGGGAGAAGATAAGTCAGCTGGGGCAGAAGGAGGTGGAGCTTCAGAAGGCTCGGAAAGAGC
  TGATCTCGAGCCAGGACACATTACAGGAGAAGCAGAGGACTCACGAGGACGCTGAGCAGCAACTACAAGC
  CTGCCAGGCTGAGAGAGCGAAGACCAAGGAGAACCTGAAAACTGAGGAGGAGCGGAGGAGGGACCTGGAC
  CAGAGGTTGACAAGCACGCGGGAGACACTAAGGCGCTTCTTCTCTGATTCATCAGACACCTGCTGTCCAT
  GCGGATGGATTCCATATCAGAAAAGGTGCTTTTACATCTCACATAACCTCAGAAGTCTGGAGGAAAGCCA
  AAAATACTGCACATCTCTGTCCTCCAAACTGGCAGCATTCAATGACTCTCCTAAGTATTCCTATCAAGTT
  TCTCTGCCCAGCGGCTTAGAGGAGTTGCTAGATCGTTCGAAGTCATATTGGATACAGGAGATGAGCAAGA
  AGTGGAGCTCATATTGTGACATGATACAAATATATAACAAGAAGTGGGAAAGAACAATTTCCAAGTGTGC
  AGAGCTTCACCCCTGCATTTGTGAGTTGGAGGCTTTCAGGTTTCCTGATGGGATCCATCTGAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR205247 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MADAITYADLRFVKVPLKNSASNHLGQDCEAYEDGELTYENVQVSPVPGGPPGLASPALADKAGVGSEQP
  TATWSSVKSSALRQIPRCPTVCLQYFLLGLLVSCLMLGVAVICLGVRYLQVSRQFQEGTRIWEATNSSLQ
  QQLREKISQLGQKEVELQKARKELISSQDTLQEKQRTHEDAEQQLQACQAERAKTKENLKTEEERRRDLD
  QRLTSTRETLRRFFSDSSDTCCPCGWIPYQKRCFYISHNLRSLEESQKYCTSLSSKLAAFNDSPKYSYQV
  SLPSGLEELLDRSKSYWIQEMSKKWSSYCDMIQIYNKKWERTISKCAELHPCICELEAFRFPDGIHLN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC003824
• ORF Size: 1044 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC003824, AAH03824
• RefSeq Size: 1423 bp
• RefSeq ORF: 1046 bp
• Locus ID: 12517
• MW: 39.7 kDa
• Gene Summary: Plays a role in B-cell proliferation and differentiation. [UniProtKB/Swiss-Prot Function]