Cd72 (BC003824) Mouse Tagged ORF Clone

CAT#: MR205247

  • TrueORF®

Cd72 (Myc-DDK-tagged) - Mouse CD72 antigen (cDNA clone MGC:6220 IMAGE:3599554)


  "BC003824" in other vectors (4)


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Reconstitution Protocol

USD 210.00

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Size
    • 10 ug

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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Cd72
Synonyms CD72c|Ly-19|Ly-32|Ly-m19|Lyb-2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR205247 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGACGCTATCACGTATGCAGACCTGCGCTTTGTGAAAGTGCCCCTGAAGAACAGTGCATCTAACC
ATCTAGGACAGGACTGTGAGGCCTATGAAGATGGGGAACTCACCTACGAGAACGTGCAAGTGTCTCCAGT
CCCAGGAGGGCCACCAGGCTTGGCTTCCCCTGCACTAGCGGACAAAGCAGGGGTCGGGTCAGAGCAACCA
ACTGCGACCTGGAGCTCTGTGAAGTCGTCTGCTCTCAGGCAGATTCCCCGCTGTCCTACAGTCTGCTTGC
AATACTTCTTGCTTGGCCTTCTCGTGTCCTGTCTGATGTTAGGGGTGGCTGTCATCTGCCTGGGAGTTCG
CTATCTGCAGGTGTCTCGGCAGTTCCAGGAGGGGACCAGGATTTGGGAAGCCACCAATAGCAGCCTGCAG
CAGCAGCTCAGGGAGAAGATAAGTCAGCTGGGGCAGAAGGAGGTGGAGCTTCAGAAGGCTCGGAAAGAGC
TGATCTCGAGCCAGGACACATTACAGGAGAAGCAGAGGACTCACGAGGACGCTGAGCAGCAACTACAAGC
CTGCCAGGCTGAGAGAGCGAAGACCAAGGAGAACCTGAAAACTGAGGAGGAGCGGAGGAGGGACCTGGAC
CAGAGGTTGACAAGCACGCGGGAGACACTAAGGCGCTTCTTCTCTGATTCATCAGACACCTGCTGTCCAT
GCGGATGGATTCCATATCAGAAAAGGTGCTTTTACATCTCACATAACCTCAGAAGTCTGGAGGAAAGCCA
AAAATACTGCACATCTCTGTCCTCCAAACTGGCAGCATTCAATGACTCTCCTAAGTATTCCTATCAAGTT
TCTCTGCCCAGCGGCTTAGAGGAGTTGCTAGATCGTTCGAAGTCATATTGGATACAGGAGATGAGCAAGA
AGTGGAGCTCATATTGTGACATGATACAAATATATAACAAGAAGTGGGAAAGAACAATTTCCAAGTGTGC
AGAGCTTCACCCCTGCATTTGTGAGTTGGAGGCTTTCAGGTTTCCTGATGGGATCCATCTGAAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR205247 protein sequence
Red=Cloning site Green=Tags(s)

MADAITYADLRFVKVPLKNSASNHLGQDCEAYEDGELTYENVQVSPVPGGPPGLASPALADKAGVGSEQP
TATWSSVKSSALRQIPRCPTVCLQYFLLGLLVSCLMLGVAVICLGVRYLQVSRQFQEGTRIWEATNSSLQ
QQLREKISQLGQKEVELQKARKELISSQDTLQEKQRTHEDAEQQLQACQAERAKTKENLKTEEERRRDLD
QRLTSTRETLRRFFSDSSDTCCPCGWIPYQKRCFYISHNLRSLEESQKYCTSLSSKLAAFNDSPKYSYQV
SLPSGLEELLDRSKSYWIQEMSKKWSSYCDMIQIYNKKWERTISKCAELHPCICELEAFRFPDGIHLN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC003824
ORF Size 1044 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq BC003824, AAH03824
RefSeq Size 1423 bp
RefSeq ORF 1046 bp
Locus ID 12517
MW 39.7 kDa
Gene Summary Plays a role in B-cell proliferation and differentiation. [UniProtKB/Swiss-Prot Function]

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