Nf2 (BC005442) Mouse Tagged ORF Clone

CAT#: MR205376

Nf2 (Myc-DDK-tagged) - Mouse neurofibromatosis 2 (cDNA clone MGC:6102 IMAGE:3496053)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nf2
• Synonyms: merlin
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR205376 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGGAGCCATCGCTTCTCGCATGAGCTTCAGCTCACTCAAGAGGAAGCAGCCCAAGACATTCACGG
  TGCGGATCGTCACCATGGACGCCGAGATGGAGTTCAACTGCGAGATGAAATGGAAGGGGAAGGACCTGTT
  TGATTTGGTGTGCCGGACACTGGGGCTTCGGGAAACCTGGTTCTTTGGACTGCAGTATACAATCAAGGAC
  ACGGTGGCCTGGCTCAAAATGGACAAGAAGGTGTTGGATCATGATGTTTCGAAGGAAGAACCAGTTACCT
  TTCACTTCCTGGCCAAATTTTATCCTGAAAATGCTGAGGAGGAGCTAGTTCAAGAGATCACGCAACACTT
  ATTTTTCTTACAGGTGAAGAAGCAGATTTTGGATGAAAAGGTCTACTGCCCTCCCGAGGCGTCCGTGCTC
  TTGGCGTCATATGCTGTCCAGGCCAAGTATGGCGACTATGACCCCTCTGTGCACAAGCGGGGATTTTTAG
  CCCAAGAGGAATTGCTCCCGAAAAGGGTGATAAATCTCTATCAGATGACTCCGGAAATGTGGGAGGAGAG
  AATTACGGCTTGGTATGCGGAGCACCGGGGCAGAGCCAGGGATGAAGCTGAAATGGAGTATTTGAAGATA
  GCTCAGGACCTGGAGATGTATGGTGTGAACTACTTTACAATCCGGAATAAAAAGGGCACAGAGTTGCTGC
  TTGGAGTGGATGCTCTTGGGCTTCATATCTATGACCCTGAGAACAGGCTGACCCCCAAGATCTCCTTCCT
  ATGGAATGAAATCCGAAACATCTCCTACAGCGACAAGGAGTTTACTATTAAACCACTGGATAAGAAAATT
  GATGTCTTCAAATTTAACTCCTCAAAGCTTCGTGTTAATAAGCTGATTCTTCAGCTATGTATTGGGAACC
  ATGACCTATTTATGAGGCGACGGAAAGCTGACTCTTTAGAAGTTCAGCAGATGAAAGCCCAGGCCAGGGA
  AGAGAGAGAGAGAGAGAGGGGGCGGGGGGGAGAGAGAGAGAGAGCTCGAATGGGTCCCCAGAATGTCTCC
  AGCCTGCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR205376 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAGAIASRMSFSSLKRKQPKTFTVRIVTMDAEMEFNCEMKWKGKDLFDLVCRTLGLRETWFFGLQYTIKD
  TVAWLKMDKKVLDHDVSKEEPVTFHFLAKFYPENAEEELVQEITQHLFFLQVKKQILDEKVYCPPEASVL
  LASYAVQAKYGDYDPSVHKRGFLAQEELLPKRVINLYQMTPEMWEERITAWYAEHRGRARDEAEMEYLKI
  AQDLEMYGVNYFTIRNKKGTELLLGVDALGLHIYDPENRLTPKISFLWNEIRNISYSDKEFTIKPLDKKI
  DVFKFNSSKLRVNKLILQLCIGNHDLFMRRRKADSLEVQQMKAQAREERERERGRGGERERARMGPQNVS
  SLL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC005442
• ORF Size: 1059 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC005442, AAH05442
• RefSeq Size: 2127 bp
• RefSeq ORF: 1061 bp
• Locus ID: 18016
• MW: 41.5 kDa