Sord (NM_146126) Mouse Tagged ORF Clone

CAT#: MR205452

Sord (Myc-DDK-tagged) - Mouse sorbitol dehydrogenase (Sord)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Sord
• Synonyms: SDH; Sdh-1; Sdh1; Sodh-1; XDH
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR205452 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCTCCAGCTAAGGGCGAGAACCTGTCCCTGGTGGTGCACGGACCTGGAGACATCCGCCTGGAGA
  ACTACCCAATACCTGAGCTCGGCCCAAATGATGTGTTACTAAAGATGCACTCGGTGGGGATTTGCGGCTC
  AGATGTTCACTACTGGGAGCATGGACGAATTGGGGACTTTGTTGTGAAAAAGCCAATGGTGCTTGGGCAT
  GAGGCTGCTGGAACAGTCACAAAAGTAGGAGAGTTGGTGAAACATCTGAAACCAGGAGATCGGGTTGCCA
  TCGAGCCTGGCGTTCCCCGAGAAGTAGATGAGTACTGCAAGATTGGCCGGTACAACCTGACGCCGACTAT
  CTTCTTCTGTGCCACGCCCCCGGATGATGGAAACCTCTGCCGATTCTACAAGCACAATGCTGACTTCTGC
  TACAAGCTTCCTGACAGTGTCACTTTTGAAGAAGGGGCCCTGATTGAACCTCTCTCTGTGGGGATCTATG
  CCTGCCGTCGAGGTTCAGTTTCCCTGGGGAACAAGGTCCTTGTGTGTGGCGCTGGGCCAGTTGGGATGGT
  CACTTTGCTTGTGGCCAAAGCAATGGGAGCTGCTCAAGTTGTGGTGACTGACCTATCCGCTTCTCGGTTG
  ACCAAAGCCAAGGAAGTTGGAGCAGACTTTACCATCCAGGTTGGCAAAGAGACCCCTCAGGAAATTGCCA
  GTAAGGTGGAAAGCCTGCTGGGAAGCAAGCCGGAGGTTACCATTGAGTGCACAGGAGCCGAGTCCTCCGT
  CCAGACGGGCATCTATGCCACTCACTCTGGTGGGACCTTGGTGATTGTGGGAATGGGCGCCGAGATGGTC
  AATTTACCCCTGGTGCACGCAGCCATTCGGGAAGTGGATATCAAAGGCGTGTTTCGATACTGCAACACGT
  GGCCGATGGCAATTTCCATGCTTGCATCGAAGACGTTGAATGTAAAGCCCTTAGTTACCCACAGATTCCC
  CCTGGAGAAGGCAGTTGAAGCCTTTGAAACAGCCAAAAAGGGAGTGGGGCTGAAAGTTATGATCAAGTGT
  GACCCCAATGACCAAAACCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR205452 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAPAKGENLSLVVHGPGDIRLENYPIPELGPNDVLLKMHSVGICGSDVHYWEHGRIGDFVVKKPMVLGH
  EAAGTVTKVGELVKHLKPGDRVAIEPGVPREVDEYCKIGRYNLTPTIFFCATPPDDGNLCRFYKHNADFC
  YKLPDSVTFEEGALIEPLSVGIYACRRGSVSLGNKVLVCGAGPVGMVTLLVAKAMGAAQVVVTDLSASRL
  TKAKEVGADFTIQVGKETPQEIASKVESLLGSKPEVTIECTGAESSVQTGIYATHSGGTLVIVGMGAEMV
  NLPLVHAAIREVDIKGVFRYCNTWPMAISMLASKTLNVKPLVTHRFPLEKAVEAFETAKKGVGLKVMIKC
  DPNDQNP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_146126
• ORF Size: 1074 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_146126.1, NM_146126.2, NM_146126.3, NM_146126.4, NP_666238.1
• RefSeq Size: 2259 bp
• RefSeq ORF: 1074 bp
• Locus ID: 20322
• MW: 38.2 kDa