Il1rap (NM_134103) Mouse Tagged ORF Clone

CAT#: MR205511

Il1rap (Myc-DDK-tagged) - Mouse interleukin 1 receptor accessory protein (Il1rap), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Il1rap
• Synonyms: 6430709H04Rik; AI255955; AV239853; IL-1RAcP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR205511 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGACTTCTGTGGTATTTGATGAGTCTGTCCTTCTATGGGATCCTGCAGAGTCATGCTTCGGAGCGCT
  GTGATGACTGGGGACTAGATACCATGCGACAAATCCAAGTGTTTGAAGATGAGCCGGCTCGAATCAAGTG
  CCCCCTCTTTGAACACTTCCTGAAGTACAACTACAGCACTGCCCATTCCTCTGGCCTTACCCTGATCTGG
  TACTGGACCAGGCAAGACCGGGACCTGGAGGAGCCCATTAACTTCCGCCTCCCAGAGAATCGCATCAGTA
  AGGAGAAAGATGTGCTCTGGTTCCGGCCCACCCTCCTCAATGACACGGGCAATTACACCTGCATGTTGAG
  GAACACAACTTACTGCAGCAAAGTTGCATTTCCCCTGGAAGTTGTTCAGAAGGACAGCTGTTTCAATTCT
  GCCATGAGATTCCCAGTGCACAAGATGTATATTGAACATGGCATTCATAAGATCACATGTCCAAATGTAG
  ACGGATACTTTCCTTCCAGTGTCAAACCATCGGTCACTTGGTATAAGGGTTGTACTGAAATAGTGGACTT
  TCATAATGTACTACCCGAGGGCATGAACTTGAGCTTTTTCATCCCCTTGGTTTCAAATAACGGAAATTAC
  ACATGTGTGGTTACATATCCTGAAAACGGACGTCTCTTTCACCTCACCAGGACTGTGACTGTAAAGGTGG
  TGGGCTCACCAAAGGATGCATTGCCACCCCAGATCTATTCTCCAAATGACCGTGTTGTCTATGAGAAAGA
  ACCAGGAGAGGAACTGGTTATTCCCTGCAAAGTCTATTTCAGTTTCATTATGGACTCCCACAATGAGGTC
  TGGTGGACCATTGATGGAAAGAAGCCTGATGACGTCACAGTCGACATCACTATTAATGAAAGTGTAAGTT
  ATTCTTCAACGGAAGATGAAACAAGGACTCAGATTTTGAGCATCAAGAAAGTCACCCCGGAGGATCTCAG
  GCGCAACTATGTCTGTCATGCTCGAAATACCAAAGGGGAAGCTGAGCAGGCTGCCAAGGTGAAACAGAAA
  GGTAATGGATGCACCGAGCCGATGACTCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR205511 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGLLWYLMSLSFYGILQSHASERCDDWGLDTMRQIQVFEDEPARIKCPLFEHFLKYNYSTAHSSGLTLIW
  YWTRQDRDLEEPINFRLPENRISKEKDVLWFRPTLLNDTGNYTCMLRNTTYCSKVAFPLEVVQKDSCFNS
  AMRFPVHKMYIEHGIHKITCPNVDGYFPSSVKPSVTWYKGCTEIVDFHNVLPEGMNLSFFIPLVSNNGNY
  TCVVTYPENGRLFHLTRTVTVKVVGSPKDALPPQIYSPNDRVVYEKEPGEELVIPCKVYFSFIMDSHNEV
  WWTIDGKKPDDVTVDITINESVSYSSTEDETRTQILSIKKVTPEDLRRNYVCHARNTKGEAEQAAKVKQK
  GNGCTEPMTL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_134103
• ORF Size: 1083 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_134103.1, NM_134103.2, NP_598864.1
• RefSeq Size: 2352 bp
• RefSeq ORF: 1083 bp
• Locus ID: 16180
• MW: 41.6 kDa