Cyp20a1 (BC049147) Mouse Tagged ORF Clone

CAT#: MR205520

Cyp20a1 (Myc-DDK-tagged) - Mouse cytochrome P450, family 20, subfamily A, polypeptide 1 (cDNA clone MGC:56832 IMAGE:6306072)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cyp20a1
• Synonyms: Cypm
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR205520 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGAAGTCATTATTAGGGTATCAGTCTGGCGGAGGGAGTGCGGGTGAGGACCACGTGAGGAGGAAAC
  TGTATGGAGATGCCGTGACAGCTTCTCTGCATAGTAACTTCCCTCTCCTGCTACAGCTTTCAGAAGAATT
  GTTAGATAAATGGCTGTCCTATCCAGAGACCCAGCACATACCTCTGAGCCAGCATATGCTCGGTTTTGCT
  TTGAAGTTTGTCACAAGGATGGTACTGGGTAGCACCTTTGAAGATGAACAGGAGGTCATCCGATTCCAAA
  AGATTCATGGCACAGTGTGGTCTGAGATTGGAAAAGGCTTTCTAGATGGGTCACTCGATAAAAACACCAC
  TCGGAAAAAACAGTATCAAGAGGCTCTCATGCAATTGGAATCCACTTTAAAGAAAATCATAAAGGAACGA
  AAAGGAGGGAACTTCAGGCAACACACCTTCATCGACTCCTTAACACAGGGAAAACTTAATGAGCAACAGA
  TCCTAGAGGACTGTGTGGTGTTCTCTCTGGCCAGCTGCATAATAACTGCAAGGTTGTGTACCTGGACGAT
  CCATTTCCTTACGACGACTGGAGAAGTTCAGAAGAAGCTATGCAAGGAGATAGACCAAGTTTTAGGGGAG
  GGTCCTATCACTTCAGAGAAAATTGAGCAGCTCAGTTATTGTCAGCAAGTACTTTTTGAAACTGTTCGGA
  CTGCTAAACTAACTCCAGTTTCTGCCCGGCTTCAAGACATTGAAGGAAAAGTCGGCCCATTTGTTATTCC
  TAAGGAGACACTCGTCCTTTATGCCCTTGGCGTGGTTCTTCAGGACCCCAGCACTTGGCCATTACCACAC
  AGGTTTGATCCAGACCGCTTTGCTGACGAGCCAGTGATGAAAGTGTTTTCTTCTCTTGGCTTCTCAGGCA
  CATGGGAATGTCCAGAACTGAGGTTTGCTTACATGGTGACCGCAGTGCTTGTGAGTGTGTTGCTGAAGAG
  GCTGCGCCTGCTCGCTGTGGACAGACAAGTTTTTGAAATGAAGTATGAACTGGTCACATCAGCAAGGGAA
  GAAGCTTGGATCACTGTCTCCAAAAGACAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR205520 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLKSLLGYQSGGGSAGEDHVRRKLYGDAVTASLHSNFPLLLQLSEELLDKWLSYPETQHIPLSQHMLGFA
  LKFVTRMVLGSTFEDEQEVIRFQKIHGTVWSEIGKGFLDGSLDKNTTRKKQYQEALMQLESTLKKIIKER
  KGGNFRQHTFIDSLTQGKLNEQQILEDCVVFSLASCIITARLCTWTIHFLTTTGEVQKKLCKEIDQVLGE
  GPITSEKIEQLSYCQQVLFETVRTAKLTPVSARLQDIEGKVGPFVIPKETLVLYALGVVLQDPSTWPLPH
  RFDPDRFADEPVMKVFSSLGFSGTWECPELRFAYMVTAVLVSVLLKRLRLLAVDRQVFEMKYELVTSARE
  EAWITVSKRH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC049147
• ORF Size: 1080 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC049147, AAH49147
• RefSeq Size: 2044 bp
• RefSeq ORF: 1082 bp
• Locus ID: 77951
• MW: 40.9 kDa