Zdhhc16 (NM_023740) Mouse Tagged ORF Clone

CAT#: MR205528

Zdhhc16 (Myc-DDK-tagged) - Mouse zinc finger, DHHC domain containing 16 (Zdhhc16)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Zdhhc16
• Synonyms: 1500015N03Rik; Aph2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR205528 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGGGCCAGCGAAGTCTGCTGTTGGGGCCAGCCCGCCTCTGCCTGCGCCTCCTTTTGCTCCTGGGCT
  ACCGGCGTCGATGCCCACCTCTGCTCCGGGGCCTGGTACAGCGCTGGCGTTATGGCAAGGTTTGCCTGCG
  CTCCCTGCTCTACAATTCCTTTGGAGGCAGTGACACTGCCGTTGATGCTGCCTTTGAGCCTGTCTACTGG
  CTGGTGGACAATGTGATCCGCTGGTTTGGAGTGGTGTTTGTGGTGCTGGTGATCGTGCTGACCGGGTCCA
  TCGTGGCCATTGCTTATCTGTGTGTCCTGCCCCTCATCCTCCGAACCTACTCGGTACCACGGCTCTGCTG
  GCATTTCTTCTATAGTCACTGGAATCTGATCCTCATCGTCTTCCATTACTACCAGGCCATCACCACTCCT
  CCTGGATACCCACCCCAGGGCAGGAATGACATTGCTACTGTCTCCATCTGTAAGAAGTGCATTTATCCCA
  AGCCAGCCCGAACACACCACCGCAGCATCTGTAATAGGTGTGTGCTGAAGATGGATCATCACTGCCCCTG
  GCTAAACAACTGTGTGGGCCACTATAACCATCGCTACTTCTTCTCTTTCTGCTTTTTCATGACTCTGGGC
  TGTGTCTACTGTAGCTATGGAAGTTGGGACCTTTTCCGGGAGGCTTATGCTGCCATTGAGACGTACCACC
  AGACTCCACCACCTACCTTCTCCTTTCGAGAAAGGATAACTCACAAGAGTCTTGTCTACCTCTGGTTCCT
  GTGCAGTTCTGTGGCCCTGGCCCTGGGTGCCCTAACCATGTGGCATGCTGTTCTCATCAGTCGGGGTGAG
  ACTAGCATCGAAAGGCATATCAACAAGAAGGAGAGGCGCCGGTTACAGGCCAAGGGCAGGGTGTTTAGGA
  ATCCTTACAACTATGGCTGCTTGGACAACTGGAAGGTGTTCCTAGGTGTGGACACAGGAAGGCATTGGCT
  GACCCGGGTGCTGTTACCTTCTAGTCACCTGCCCCATGGGAATGGGATGAGCTGGGACCCTCCTCCCTGG
  GTGACTGCTCACTCAGCCTCTGTGATGGCCGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR205528 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRGQRSLLLGPARLCLRLLLLLGYRRRCPPLLRGLVQRWRYGKVCLRSLLYNSFGGSDTAVDAAFEPVYW
  LVDNVIRWFGVVFVVLVIVLTGSIVAIAYLCVLPLILRTYSVPRLCWHFFYSHWNLILIVFHYYQAITTP
  PGYPPQGRNDIATVSICKKCIYPKPARTHHRSICNRCVLKMDHHCPWLNNCVGHYNHRYFFSFCFFMTLG
  CVYCSYGSWDLFREAYAAIETYHQTPPPTFSFRERITHKSLVYLWFLCSSVALALGALTMWHAVLISRGE
  TSIERHINKKERRRLQAKGRVFRNPYNYGCLDNWKVFLGVDTGRHWLTRVLLPSSHLPHGNGMSWDPPPW
  VTAHSASVMAV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_023740
• ORF Size: 1086 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_023740.1, NM_023740.2, NP_076229.2
• RefSeq Size: 1838 bp
• RefSeq ORF: 1086 bp
• Locus ID: 74168
• MW: 41.8 kDa