Lefty1 (NM_010094) Mouse Tagged ORF Clone

CAT#: MR205659

Lefty1 (Myc-DDK-tagged) - Mouse left right determination factor 1 (Lefty1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Lefty1
• Synonyms: AI450052; Ebaf; Leftb; lefty; lefty-1; Stra3; Tgfb4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR205659 representing NM_010094
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCATTCCTGTGGCTCTGCTGGGCACTCTGGGCACTGTCGCTGGTTAGCCTCAGGGAAGCCCTGACCG
  GAGAGCAGATCCTGGGCAGCCTGCTGCAACAGCTGCAGCTCGATCAACCGCCAGTCCTGGACAAGGCTGA
  TGTGGAAGGGATGGTCATCCCCTCGCACGTGAGGACTCAGTATGTGGCCCTGCTACAACACAGCCATGCC
  AGCCGCTCCCGAGGCAAGAGGTTCAGCCAGAACCTTCGAGAGGTGGCAGGCAGGTTCCTGGTGTCAGAGA
  CCTCCACTCACCTGCTAGTGTTCGGAATGGAGCAGCGGCTGCCGCCTAACAGCGAGCTGGTGCAGGCTGT
  GCTGCGGCTGTTCCAGGAGCCTGTGCCCAGAACAGCTCTCCGGAGGCAAAAGAGGCTGTCCCCACACAGT
  GCCCGGGCTCGGGTCACCATTGAATGGCTGCGCTTCCGCGACGACGGCTCCAACCGCACTGCCCTTATCG
  ATTCTAGGCTCGTGTCCATCCACGAGAGCGGCTGGAAGGCCTTCGACGTGACCGAGGCCGTGAACTTCTG
  GCAGCAGCTGAGCCGGCCGAGGCAGCCGCTGCTGCTCCAGGTGTCGGTGCAGAGGGAGCATCTGGGGCCG
  GGAACCTGGAGCTCACACAAGTTGGTTCGTTTCGCGGCGCAGGGGACGCCGGATGGCAAGGGGCAGGGCG
  AGCCACAGCTGGAGCTGCACACGCTGGACCTCAAGGACTATGGAGCTCAAGGCAATTGTGACCCCGAGGC
  ACCAGTGACTGAAGGCACCCGATGCTGTCGCCAGGAGATGTACCTGGACCTGCAGGGGATGAAGTGGGCC
  GAGAACTGGATCCTAGAACCGCCAGGGTTCCTGACATATGAATGTGTGGGCAGCTGCCTGCAGCTACCGG
  AGTCCCTGACCAGCAGGTGGCCATTTCTGGGGCCTCGGCAGTGTGTCGCCTCAGAGATGACCTCCCTGCC
  CATGATTGTCAGCGTGAAGGAGGGAGGCAGGACCAGGCCTCAAGTGGTCAGCCTGCCCAACATGAGGGTG
  CAGACCTGTAGCTGCGCCTCAGATGGGGCGCTCATACCCAGGAGGCTGCAGCCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR205659 representing NM_010094
  Red=Cloning site Green=Tags(s)
  
  MPFLWLCWALWALSLVSLREALTGEQILGSLLQQLQLDQPPVLDKADVEGMVIPSHVRTQYVALLQHSHA
  SRSRGKRFSQNLREVAGRFLVSETSTHLLVFGMEQRLPPNSELVQAVLRLFQEPVPRTALRRQKRLSPHS
  ARARVTIEWLRFRDDGSNRTALIDSRLVSIHESGWKAFDVTEAVNFWQQLSRPRQPLLLQVSVQREHLGP
  GTWSSHKLVRFAAQGTPDGKGQGEPQLELHTLDLKDYGAQGNCDPEAPVTEGTRCCRQEMYLDLQGMKWA
  ENWILEPPGFLTYECVGSCLQLPESLTSRWPFLGPRQCVASEMTSLPMIVSVKEGGRTRPQVVSLPNMRV
  QTCSCASDGALIPRRLQP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010094
• ORF Size: 1104 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_010094.1, NM_010094.2, NM_010094.3, NM_010094.4, NP_034224.1
• RefSeq Size: 1622
• RefSeq ORF: 1107
• Locus ID: 13590
• MW: 41.9 kDa
• Gene Summary: This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate the mature protein, which plays a role in left-right asymmetry determination of organ systems during development. Mice lacking a functional copy of this gene exhibit embryonic lethality and defects in left-right patterning. [provided by RefSeq, Aug 2016]