Kcnj1 (NM_019659) Mouse Tagged ORF Clone

CAT#: MR205751

Kcnj1 (Myc-DDK-tagged) - Mouse potassium inwardly-rectifying channel, subfamily J, member 1 (Kcnj1), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Kcnj1
• Synonyms: Kir1.1; ROMK; Romk2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR205751 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCAAACATCTTCGAAGATGGTTTGTCACTCACATATTTGGGCGTTCTCGGCAACGAGCAAGGTTGG
  TCTCCAAAGATGGAAGGTGTAACATCGAGTTTGGCAATGTAGATGCACAGTCGAGGTTTATATTCTTTGT
  GGATATCTGGACAACTGTACTTGACCTGAAATGGAGGTACAAAATGACCGTGTTCATCACAGCCTTCTTG
  GGGAGTTGGTTTCTCTTTGGTCTCCTGTGGTATGTCGTAGCCTATGTTCATAAGGATCTCCCAGAGTTCT
  ACCCACCTGACAACCGTACTCCTTGTGTGGAGAACATTAATGGCATGACATCAGCCTTTCTGTTTTCTCT
  AGAGACCCAAGTGACCATAGGTTACGGATTCAGGTTTGTGACAGAACAGTGTGCCACTGCCATTTTTCTG
  CTTATCTTCCAGTCTATTCTTGGAGTGATCATCAATTCTTTCATGTGTGGTGCCATATTAGCCAAGATCT
  CTAGACCCAAAAAACGTGCAAAGACCATTACATTCAGCAAGAATGCGGTGATCAGCAAACGTGGGGGGAA
  GCTCTGTCTCCTCATCCGAGTAGCAAATCTTAGGAAAAGCCTTCTGATTGGCAGTCACATATATGGTAAG
  CTTCTGAAGACTACCATCACACCTGAAGGAGAGACCATTATTTTGGATCAGACCAATATAAACTTTGTAG
  TTGATGCTGGCAATGAAAATTTGTTCTTCATTTCCCCACTGACAATCTACCACATTATTGACCACAACAG
  CCCTTTCTTCCACATGGCGGCAGAAACTCTTTCCCAACAGGACTTCGAGTTGGTTGTCTTTTTAGATGGC
  ACAGTAGAATCCACCAGTGCAACCTGCCAAGTCCGCACATCATACATCCCAGAAGAGGTGCTTTGGGGTT
  ACCGTTTTGTTCCCATCGTATCCAAGACCAAGGAAGGGAAATACCGAGTGGATTTCCATAACTTTGGTAA
  GACGGTGGAAGTGGAGACCCCTCATTGTGCCATGTGCCTCTATAATGAGAAAGATGCCAGGGCCAGGATG
  AAGAGAGGCTATGACAACCCTAACTTTGTCTTGTCAGAAGTTGATGAAACAGACGACACCCAAATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR205751 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MFKHLRRWFVTHIFGRSRQRARLVSKDGRCNIEFGNVDAQSRFIFFVDIWTTVLDLKWRYKMTVFITAFL
  GSWFLFGLLWYVVAYVHKDLPEFYPPDNRTPCVENINGMTSAFLFSLETQVTIGYGFRFVTEQCATAIFL
  LIFQSILGVIINSFMCGAILAKISRPKKRAKTITFSKNAVISKRGGKLCLLIRVANLRKSLLIGSHIYGK
  LLKTTITPEGETIILDQTNINFVVDAGNENLFFISPLTIYHIIDHNSPFFHMAAETLSQQDFELVVFLDG
  TVESTSATCQVRTSYIPEEVLWGYRFVPIVSKTKEGKYRVDFHNFGKTVEVETPHCAMCLYNEKDARARM
  KRGYDNPNFVLSEVDETDDTQM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019659
• ORF Size: 1119 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019659.1, NM_019659.2, NM_019659.3, NP_062633.1
• RefSeq Size: 3073 bp
• RefSeq ORF: 1119 bp
• Locus ID: 56379
• MW: 42.8 kDa