Aspn (NM_025711) Mouse Tagged ORF Clone
CAT#: MR205760
- TrueORF®
Aspn (Myc-DDK-tagged) - Mouse asporin (Aspn), transcript variant 1
"NM_025711" in other vectors (6)
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Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Aspn |
Synonyms | 4631401G09Rik; AA986886; Plap1; Slrr1c |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR205760 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGAGTATGTGATGCTACTGCTTTTGGCTGTGTGCTCTGCCAAACCCTTCTTTAGCCCTTCCCACA CAGCACTGAAGAATATGATGTTGAAGGATATGGAAGACACAGATGATGACGATAACGATGATGACGACAA CTCTCTTTTTCCAACGAAAGAGCCAGTGAACCCTTTTTTCCCTTTCGATTTGTTTCCAACATGTCCATTT GGGTGCCAATGTTACTCTCGAGTTGTTCACTGCTCTGATCTAGGTCTGACATCGGTTCCAAACAACATTC CATTTGATACTCGAATGGTTGACCTTCAAAATAATAAAATCAAGGAAATTAAAGAAAATGACTTTAAAGG ACTCACTTCACTTTATGCTCTGATTCTGAACAACAACAAGCTAACAAAGATTCACCCAAAAACCTTTCTA ACCACAAAGAAATTGAGAAGGCTATATTTATCCCACAACCAACTAAGTGAAATTCCACTTAATCTTCCCA AATCATTAGCAGAACTCAGAATTCATGATAATAAAGTTAAGAAGATACAAAAGGACACGTTCAAGGGAAT GAATGCTTTACATGTTTTGGAAATGAGTGCAAACCCTCTTGAGAACAACGGGATAGAACCAGGGGCATTT GAAGGGGTGACAGTATTCCATATCAGGATCGCTGAAGCAAAACTAACCTCAATTCCAAAAGGCCTACCAC CAACTTTGCTGGAGCTTCATTTAGATTTTAATAAAATTTCAACGGTGGAACTTGAAGATCTTAAACGGTA CAGGGAACTGCAAAGGCTGGGTCTTGGAAACAACAGAATCACAGATATTGAAAATGGAACTTTTGCTAAT ATACCACGTGTGAGAGAGATACACTTGGAACACAATAAACTAAAAAAAATCCCTTCAGGATTACAGGAGT TGAAATACCTCCAGATAATCTTCCTTCATTATAATTCAATTGCAAAAGTGGGAGTGAATGACTTCTGTCC AACAGTGCCAAAGATGAAGAAATCTTTATACAGTGCAATAAGTTTATTCAACAACCCAATGAAGTACTGG GAAATACAACCTGCAACATTTCGTTGTGTTCTTGGCAGAATGAGTGTTCAGCTTGGGAATGTTGGAAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR205760 protein sequence
Red=Cloning site Green=Tags(s) MKEYVMLLLLAVCSAKPFFSPSHTALKNMMLKDMEDTDDDDNDDDDNSLFPTKEPVNPFFPFDLFPTCPF GCQCYSRVVHCSDLGLTSVPNNIPFDTRMVDLQNNKIKEIKENDFKGLTSLYALILNNNKLTKIHPKTFL TTKKLRRLYLSHNQLSEIPLNLPKSLAELRIHDNKVKKIQKDTFKGMNALHVLEMSANPLENNGIEPGAF EGVTVFHIRIAEAKLTSIPKGLPPTLLELHLDFNKISTVELEDLKRYRELQRLGLGNNRITDIENGTFAN IPRVREIHLEHNKLKKIPSGLQELKYLQIIFLHYNSIAKVGVNDFCPTVPKMKKSLYSAISLFNNPMKYW EIQPATFRCVLGRMSVQLGNVGK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_025711 |
ORF Size | 1122 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_025711.2, NM_025711.3, NP_079987.2 |
RefSeq Size | 2362 bp |
RefSeq ORF | 1122 bp |
Locus ID | 66695 |
MW | 42.6 kDa |
Gene Summary | This gene encodes a member of the small leucine-rich proteoglycan family. The encoded protein is an extracellular matrix protein that modulates the transforming growth factor-beta signaling pathway, regulating cartilage matrix gene expression and cartilage formation. The protein plays a role in the pathology of osteoarthritis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC207593 | Aspn (untagged) - Mouse asporin (Aspn), transcript variant 1, (10ug) |
USD 420.00 |
|
MG205760 | Aspn (GFP-tagged) - Mouse asporin (Aspn) |
USD 330.00 |
|
MR205760L1 | Lenti ORF clone of Aspn (Myc-DDK-tagged) - Mouse asporin (Aspn), transcript variant 1 |
USD 500.00 |
|
MR205760L2 | Lenti ORF clone of Aspn (mGFP-tagged) - Mouse asporin (Aspn), transcript variant 1 |
USD 624.00 |
|
MR205760L3 | Lenti ORF clone of Aspn (Myc-DDK-tagged) - Mouse asporin (Aspn), transcript variant 1 |
USD 624.00 |
|
MR205760L4 | Lenti ORF clone of Aspn (mGFP-tagged) - Mouse asporin (Aspn), transcript variant 1 |
USD 500.00 |
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