Kcnj15 (NM_019664) Mouse Tagged ORF Clone

CAT#: MR205834

Kcnj15 (Myc-DDK-tagged) - Mouse potassium inwardly-rectifying channel, subfamily J, member 15 (Kcnj15), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Kcnj15
• Synonyms: 4930414N08Rik; AI182284; AI267127; IRKK; Kir4.2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR205834 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGCCATTCACCTTGGCATGTCCAGTGCCCCACTGGTGAAGCATACCAACGGGGTTGGACTCAAGG
  CCCACAGACCCCGAGTCATGTCAAAGAGTGGGCACAGTAATGTGAGAATCGATAAGGTAGACGGAATCTA
  TTTACTCTACCTCCAGGACTTGTGGACAACCGTCATCGACATGAAGTGGCGATACAAGCTCACCCTATTT
  GCTGCCACCTTTGTGATGACCTGGTTTCTGTTTGGAGTGGTCTACTATGCCATAGCCTTTATTCATGGTG
  ACTTACAACTTGGGGAATCTAATTCCAACCACACACCCTGCATTATGAAAGTGGACTCTCTCACAGGAGC
  ATTCCTCTTTTCCTTGGAATCTCAGACAACCATTGGCTACGGGGTCCGTTCCATCACAGAGGAGTGTCCC
  CATGCTATCTTCCTCTTAGTCGCCCAACTGGTCATCACCACATTGATTGAGATCTTCATTACGGGGACCT
  TTCTGGCTAAAATTGCAAGACCCAAAAAGCGAGCCGAGACCATTAAGTTCAGCCACTGTGCTGTCATCAG
  CAAGCAGAATGGAAAGCTATGCCTGGTCATCCAGGTGGCCAACATGAGGAAGAGTCTCCTGATTCAGTGC
  CAGCTCTCTGGAAAACTCCTGCAGACACACGTCACCAAAGAGGGAGAACGCATTCTCCTCAACCAGGCCA
  CTGTCAAATTCCACGTGGACTCCTCTTCCGAGAGTCCCTTCCTCATCCTGCCCATGACCTTCTACCACGT
  GTTGGATGAGACAAGCCCCCTGCGGGACCTCACACCCCAAAACCTAAAGGAGAAGGAGTTTGAGCTGGTG
  GTACTTCTCAACGCCACGGTGGAGTCTACCAGCGCCGTCTGCCAGAGCCGAACGTCTTACATCCCGGAGG
  AGATCTACTGGGGCTTTGAGTTTGTGCCTGTGGTTTCTCTCTCCAAAAATGGAAAGTATGTGGCTGATTT
  CAGTCAATTTGAGCAGATCAGGAAGAGCCCGGATTGTACCTTCTACTGTGCCGATTCTGAGAAGCAGAAG
  CTTGAAGAACAGTACAGGCAAGAGGACCAGAGGGAGCGGGAGCTGAGGAGCCTCCTGCTACAGCAGAGCA
  ATGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR205834 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDAIHLGMSSAPLVKHTNGVGLKAHRPRVMSKSGHSNVRIDKVDGIYLLYLQDLWTTVIDMKWRYKLTLF
  AATFVMTWFLFGVVYYAIAFIHGDLQLGESNSNHTPCIMKVDSLTGAFLFSLESQTTIGYGVRSITEECP
  HAIFLLVAQLVITTLIEIFITGTFLAKIARPKKRAETIKFSHCAVISKQNGKLCLVIQVANMRKSLLIQC
  QLSGKLLQTHVTKEGERILLNQATVKFHVDSSSESPFLILPMTFYHVLDETSPLRDLTPQNLKEKEFELV
  VLLNATVESTSAVCQSRTSYIPEEIYWGFEFVPVVSLSKNGKYVADFSQFEQIRKSPDCTFYCADSEKQK
  LEEQYRQEDQRERELRSLLLQQSNV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019664
• ORF Size: 1128 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_019664.1, NM_019664.2, NM_019664.3, NM_019664.4, NM_019664.5, NP_062638.1
• RefSeq Size: 5222
• RefSeq ORF: 1128
• Locus ID: 16516
• MW: 42.6 kDa
• Gene Summary: Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. [UniProtKB/Swiss-Prot Function]