Psmd4 (BC009005) Mouse Tagged ORF Clone

CAT#: MR205907

Psmd4 (Myc-DDK-tagged) - Mouse proteasome (prosome, macropain) 26S subunit, non-ATPase, 4 (cDNA clone MGC:6683 IMAGE:3581937), complete

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Psmd4
• Synonyms: Mcb1, angiocidin
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR205907 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGTTGGAGAGCACTATGGTTTGTGTGGACAACAGTGAGTACATGCGGAACGGAGACTTCCTTCCCA
  CCCGGCTGCAGGCCCAGCAGGATGCCGTCAACATTGTATGTCACTCAAAGACCCGAAGCAACCCTGAGAA
  TAACGTGGGCCTGATCACACTGGCCAATGACTGTGAGGTGCTGACCACACTCACCCCGGACACTGGCCGT
  ATCCTCTCCAAGCTCCACACTGTCCAACCCAAAGGCAAGATCACCTTCTGCACTGGCATCCGCGTGGCCC
  ACTTGGCTCTGAAGCACCGGCAGGGCAAGAATCACAAGATGCGCATCATCGCCTTTGTCGGTAGCCCTGT
  GGAGGACAACGAGAAGGATCTGGTGAAACTAGCTAAACGCCTTAAGAAAGAAAAAGTGAATGTTGACATC
  ATTAATTTTGGGGAAGAGGAGGTGAACACAGAGAAGCTGACAGCCTTTGTGAACACACTGAATGGCAAGG
  ATGGAACTGGGTCCCATCTAGTGACAGTGCCTCCTGGACCTAGCTTGGCTGATGCTCTCATCAGTTCTCC
  TATTCTGGCTGGTGAAGGCGGTGCCATGCTGGGTCTTGGTGCCAGTGACTTTGAGTTTGGAGTAGATCCC
  AGTGCTGATCCTGAATTGGCCCTGGCCCTTCGAGTCTCTATGGAAGAGCAGCGGCAGCGGCAGGAGGAAG
  AGGCACGGCGGGCCGCTGCGGCCTCTGCAGCTGAGGCTGGAATTGCTACACCTGGGACTGAAGGTGAAAG
  AGACTCGGATGACGCCCTACTGAAGATGACCATCAACCAGCAGGAGTTTGGCCGTCCTGGGCTTCCAGAC
  CTAAGCAGCATGACTGAGGAAGAGCAGATCGCCTACGCCATGCAGATGTCCCTGCAGGGAACAGAGTTTA
  GCCAAGAATCGGCTGACATGGATGCCAGCTCAGCCATGGACACATCTGATCCAGTCAAGGAGGAGGATGA
  CTATGACGTCATGCAGGACCCGGAGTTCCTTCAGAGCGTCCTAGAGAACCTTCCAGGTGTGGATCCCAAC
  AATGCAGCCATTCGAAGTGTCATGGGGGCTCTGGCCTCCCAGGCCACCAAGGATGGCAAGAATGACAAGA
  AAGAGGAAGAGAAGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR205907 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVLESTMVCVDNSEYMRNGDFLPTRLQAQQDAVNIVCHSKTRSNPENNVGLITLANDCEVLTTLTPDTGR
  ILSKLHTVQPKGKITFCTGIRVAHLALKHRQGKNHKMRIIAFVGSPVEDNEKDLVKLAKRLKKEKVNVDI
  INFGEEEVNTEKLTAFVNTLNGKDGTGSHLVTVPPGPSLADALISSPILAGEGGAMLGLGASDFEFGVDP
  SADPELALALRVSMEEQRQRQEEEARRAAAASAAEAGIATPGTEGERDSDDALLKMTINQQEFGRPGLPD
  LSSMTEEEQIAYAMQMSLQGTEFSQESADMDASSAMDTSDPVKEEDDYDVMQDPEFLQSVLENLPGVDPN
  NAAIRSVMGALASQATKDGKNDKKEEEKK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC009005
• ORF Size: 1137 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC009005, AAH09005
• RefSeq Size: 1277 bp
• RefSeq ORF: 1139 bp
• Locus ID: 19185
• MW: 41 kDa
• Gene Summary: Binds and presumably selects ubiquitin-conjugates for destruction. [UniProtKB/Swiss-Prot Function]