B4galt6 (NM_019737) Mouse Tagged ORF Clone

CAT#: MR205973

B4galt6 (Myc-DDK-tagged) - Mouse UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 6 (B4galt6)

Product Images

Western validation with an anti-DDK antibody; L: Control HEK293 lysate R: Over-expression lysate

• 

  Coomassie blue staining of purified B4galt6 protein (Cat# TP505973). The protein was produced from HEK293T cells transfected with B4galt6 cDNA clone (Cat# MR205973) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: B4galt6
• Synonyms: AA536803; AU022389
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR205973 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGCGCTCAAGCGGATGATGCGGGTCTCCAATCGCTCTCTGATTGCCTTCATCTTCTTCTTCTCCC
  TCTCCACGTCCTGTCTCTACTTCATCTATGTGGCTCCAGGCATCGCCAACACGTACCTCTTTATGGTACA
  AGCTAGAGGTATCATGCTGAGAGAGAATGTGAAAACAATTGGACACATGATCAGGCTGTATACCAATAAA
  AACACTACGCTCAACGGAACAGATTATCCTGAAGGCAATAATACAAGTGACTATCTTGTTCAAACAACAA
  CGTATCTTCCGCAAAACTTCACGTATTTGCCACACCTCCCCTGTCCAGAGAAGCTGCCTTACATGCGGGG
  ATTCCTCAGTGTCAATGTGAGTGAGATCAGTTTTGATGAAGTCCATCAGCTCTTCTCCAAGGACTCAGAG
  ATTGGGCCAGGAGGCCACTGGAGGCCCAAAGACTGCAAGCCCAGATGGAAGGTGGCAGTTCTCATCCCCT
  TCCGTAACCGCCATGAACATCTTCCGATTTTTTTCCTGCACCTGATTCCGATGCTCCAGAAACAGCGGCT
  GGAATTTGCCTTTTATGTCATCGAACAGACCGGCACACAACCATTCAATCGTGCGATGCTCTTTAATGTG
  GGTTTCAAAGAGGCCATGAAAGACAGAGCCTGGGACTGTGTGATCTTCCATGATGTGGATCATCTGCCTG
  AAAATGATCGGAACTACTACGGATGTGGAGAGATGCCGCGGCACTTTGCAGCGAAGCTGGACAAGTACAT
  GTACATTCTTCCCTATAAGGAGTTTTTTGGTGGGGTGAGTGGCCTGACGGTGGAGCAATTCAGGAAGATC
  AACGGCTTTCCCAATGCCTTCTGGGGATGGGGAGGCGAGGACGATGACCTTTGGAACAGAGTTCACTACG
  CCGGCTACAATGTAACCCGGCCAGAAGGGGACTTGGGAAAATACATATCTATTCCTCATCACCATCGAGG
  GGAGGTACAGTTCCTAGGACGGTATAAATTACTTCGGTATTCCAAGGAGCGGCAGTACATCGATGGACTG
  AACAATTTATTATACACACCCAAAATACTGGTTGATAGGCTGTATACAAATATATCTGTAAACCTCATGC
  CAGAATTAGCTCCAATTGAAGACTAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR205973 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSALKRMMRVSNRSLIAFIFFFSLSTSCLYFIYVAPGIANTYLFMVQARGIMLRENVKTIGHMIRLYTNK
  NTTLNGTDYPEGNNTSDYLVQTTTYLPQNFTYLPHLPCPEKLPYMRGFLSVNVSEISFDEVHQLFSKDSE
  IGPGGHWRPKDCKPRWKVAVLIPFRNRHEHLPIFFLHLIPMLQKQRLEFAFYVIEQTGTQPFNRAMLFNV
  GFKEAMKDRAWDCVIFHDVDHLPENDRNYYGCGEMPRHFAAKLDKYMYILPYKEFFGGVSGLTVEQFRKI
  NGFPNAFWGWGGEDDDLWNRVHYAGYNVTRPEGDLGKYISIPHHHRGEVQFLGRYKLLRYSKERQYIDGL
  NNLLYTPKILVDRLYTNISVNLMPELAPIEDY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019737
• ORF Size: 1149 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019737.1, NM_019737.2, NP_062711.1
• RefSeq Size: 5808
• RefSeq ORF: 1149
• Locus ID: 56386
• MW: 44.8 kDa